Abstract: The BLEC1 gene (C-type lectin-like receptor 1)encodes a C-type lectin-like receptors that is associated with the activated antigen of the CD69 family and is closely linked to the innate immune function of poultry. In order to explore the genetic diversity of BLEC1 gene and different disease resistance of different chicken breeds. We used phenol and chloroform to extract the DNA of 26 different chicken breeds. Gallus gallus as a reference, the serial number was NC_006103.4, target sequence capture assay and MEGA 6 software was used to detect SNPs, Indels and amino acid sites in the BLEC1 gene of 26 chicken breeds. Finally, MEGA 6 software was used to construct phylogenetic trees. The results showed that 14 SNPs and 12 Indels loci were detected in 26 chicken breeds, of which 5 SNPs and 3 Indels loci were exon, 3 Indels sites on exons are widely present in chicken breeds. Indel1 was presented in all chicken breeds excepted Black Wolf Pheasant. These led to a great change in amino acids. In exons, 3 amino acids missense mutations, the rest of synonymous mutations. The neighbour joining cluster analysis was used to classify 26 chicken breeds into three groups, Anka chicken, SPF-Lai Hang chicken and Taihewugu chicken grouped together, Xiaoshan chicken alone for a categorize, the rest of the breeds were grouped together.All these suggested that BLEC1 gene was rich in genetic diversity in local chicken breeds in China and it was associated with innate immunity and disease resistance of different chicken.

Key words: Chickens, BLEC1 gene, SNPs, Indels, Phylogenetic tree

CLC Number: 

• Q78

• S831