Polymorphism of Adipocyte Fatty Acid-binding Protein Gene （A-FABP） in Yak （ Bos grunniens ）

doi:10.3969/j.issn.1000-7091.2012.04.008

Abstract

Abstract: Adipocyte fatty acid-binding protein（ A-FABP）, as a member of the fatty acid binding protein（ FAB- Ps） family,play a pivotal role in regulating intracellular fat concentration and then affect intramuscular fat（IMF） in mammalian. So A-FABP was suggested as a candidate gene of IMF in some specie,s of livestock and poultry. In this study, single nucleotide polymorphisms （SNPs） was investigated at A-FABP intron 3, exon4 and 3＇-UTR in Gannan yak, Qinghai yak and Tianzhu white yak（Bos grunniens） by polymerase chain reaction-single strand conformational polymorphism（PCR-SSCP） so as to analyse their molecular genetic characteristics. Five novel SSCP patterns,repre- senting five different alleles A-E were identified in three yak populations. With alignment of the bovine （ Bos tauras） and yak A-FABP allelic sequences, six SNPs including a synonymous mutation （ c. 4222A 〉 G） at exon 4 were checked in yak. SNP c. ＊ 94T 〉 A in 3＇-UTR of A-FABP which was only detected in yak population represented one of genetic characteristics of yak that differ from the bovine. Alleles distributed disproportionably among three yak populations, such as only alleles A-C were observed in Tianzhu white yak and alleles A-D in Qinghai yak. This may be related to geographical distribution and breeding methods of different yak populations. The mutations in amplified region of yak A-FABP would become a potential locus for genetic markers of meat quality traits of yak as it showed moderate polymorphism with PIC in the range of 0.29 -0.36 and higher effective number of alleles（Ne）.

Key words: Yak, Adipocyte fatty ac ＇.d-binding protein gene （A-FABP）, PCR-SSCP SNPs

CLC Number:

S823.8

CAO Jian, LUO Yu-zhu, HU Jiang, CHENG Shu-ru, YANG Guo, LIU Xiu. Polymorphism of Adipocyte Fatty Acid-binding Protein Gene （A-FABP） in Yak （ Bos grunniens ）[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 42-47. doi: 10.3969/j.issn.1000-7091.2012.04.008.

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