Analysis of KDM7A Expression Profile in Yak and Its Expression in the Process of Oocyte Meiosis

doi:10.7668/hbnxb.20191191

Abstract

Abstract: The objective of this research was to explore the expression pattern and molecular mechanism of histone demethylase 7A(KDM7A) in the reproductive development of yak.Yak was used as research object. The coding sequence of KDM7A gene was cloned by RT-PCR. The protein structure and function of KDM7A were analyzed by bioinformatics software, and the KDM7A gene was detected by Real-time quantitative PCR (RT-qPCR)in theovary, kidney,stomach,small intestine,muscle, heart, uterus, spleen, lung and liver tissue. The expression pattern of KDM7A mRNA was analyzed during the meiotic maturation of yak oocytes.The 2 704 bp KDM7A gene of the yak was cloned, and the CDS was 2 409 bp in length and encoded a total of 802 amino acids. Compared with other species, the base sequence of yak KDM7A was higher homology with cattle, bison and goat. Yak KDM7A gene was widely expressed in various tissues,in which the uterus KDM7A mRNA relative expression was the highest and significantly higher than other tissues except the stomach (P<0.05), while the relative expression of KDM7A in kidney and muscle tissue was significantly lower than that in other tissues (P<0.05).There was spatiotemporal dynamic expression feature of the KDM7A gene during the meiosis of oocytes. The expression level of KDM7A in meiosis metaphase Ⅱ (MⅡ) stage was significantly higher than that in meiosis metaphase Ⅰ(MⅠ) and germinal vesicle (GV) stages of yak oocytes (P<0.05).In summary, this study suggested that KDM7A gene was highly conserved in genetic evolution and involved in meiosis process of oocytes, which had a dynamic expression pattern during the reproductive development of yak. This study provided relevant basis for further exploring the action mechanism of yak KDM7A in the process of reproduction.

Key words: Yak, Histone demethylase, KDM7A, Oocyte, Tissue expression profile

CLC Number:

S823.8+5

HAI Zhuo, XIONG Xianrong, MA Hongcheng, HUANG Xiangyue, MIN Xingyu, LI Jian. Analysis of KDM7A Expression Profile in Yak and Its Expression in the Process of Oocyte Meiosis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 217-224. doi: 10.7668/hbnxb.20191191.

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