Cloning of AQP2 Gene and Its Expression Analysis in Male Reproductive System of Yak

doi:10.7668/hbnxb.20191334

Abstract

Abstract: The aim of this research was to clone the aquaporin 2 (AQP2) gene, identify its expression pattern in various tissues, and to analyze the expression in different growth periods of the male reproductive system in yak.This research may provide important foundation for exploration the role of AQP2 for reproductive development in yak.The samples of yak kidney, testis, epididymis, spleen, brain, lung, heart and liver were collected after slaughtering. The total RNA of different samples were extracted and the coding sequence of AQP2 gene was cloned by RT-PCR. Meanwhile the function and structure of AQP2 gene were analyzed by bioinformatics softwares.Then, the mRNA expression of AQP2 in different tissues and in different growth periods of the male reproductive system was detected by quantitative real-time PCR (qRT-PCR). The results showed that the CDS region of AQP2 gene was 816 bp, encoding 271 amino acids. It had high homology with cattle, buffalo and goat. The tissues expression analysis showed that AQP2 highly expressed in testis and kidney, which was significantly higher than other tissues (P<0.05). The results of immunohistochemistry indicated that AQP2 was expressed in round spermatids of seminiferous tubules, but no expression was found in spermatogonia, spermatocytes, elongated spermatids, leydig cells and sertoli cells. The results of qRT-PCR was found that the expression of AQP2 in vas deferens was the highest(P<0.05). The expression trend of AQP2 mRNA in testis and vas deferens was increased with age (P<0.05). While the expression level in prostate was slightly decreased with age, but the difference was not significant(P>0.05). The above results indicated that AQP2 was highly conserved during evolution, and it was highly expressed in testis and kidney. It was involved in sperm maturation and transportation, which might be completed by water reabsorption and liquid formation.

Key words: Yak, AQP2, Testis, Tissue expression

CLC Number:

Q78
S823

HUANG Xiangyue, XIONG Xianrong, HAI Zhuo, MU Songyin, LI Jian. Cloning of AQP2 Gene and Its Expression Analysis in Male Reproductive System of Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 225-231. doi: 10.7668/hbnxb.20191334.

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