Cloning of Yak AQP8 and Expression Analysis in Various Tissues and Liver at Different Growth Stages

doi:10.7668/hbnxb.20191419

Abstract

Abstract: In order to identify the AQP8 gene of yak, analyze its expression differences in different tissues and livers at different growth stages, and explore the differences of expression and distribution of AQP8 protein, the CDS of yak AQP8 gene was cloned by RT-PCR and then the sequence was analyzed using bioinformatics. The RT-qPCR was used to detect the mRNA expression level of AQP8 in 8 tissues and liver at different growth stages. Immunohistochemistry was used to study the expression and distribution of livers in different ages. The results showed that the ORF of AQP8 gene was 792 bp, encoding 263 amino acids. The phylogenetic tree analysis showed that the AQP8 gene of Maiwa yak had the closest associated with that of Bos mutus. The AQP8 protein has a stable MIP domain specific to AQPs. The mRNA expression level of AQP8 in liver of adult yak was significantly higher than heart, spleen, kidney and other tissues(P<0.05), and the expression level of AQP8 in liver at juvenile(15 months) was significantly higher than fetus(1 day) and adult(5 years) (P<0.05). Immunohistochemical results showed that AQP8 protein expression was the highest in liver of juvenile(15 months) yak(P<0.05). This study provided relevant basis for further exploring the mechanism of yak AQP8 in the growth process, and provided implications for study of mammals in the alpine and hypoxic environment.

Key words: Yak, Aquaporin 8, Gene cloning, Tissue expression, Liver

CLC Number:

Q78
S823

LI Juan, WANG Li, LUO Xiaolin, GUAN Jiuqiang. Cloning of Yak AQP8 and Expression Analysis in Various Tissues and Liver at Different Growth Stages[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 219-224. doi: 10.7668/hbnxb.20191419.

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