Cloning of Yak HDAC2 Gene and Its Expression Pattern in Testis

doi:10.7668/hbnxb.20191525

Abstract

Abstract: The aim of this research is to clone the HDAC2 gene in yak, predict the structure and property of HDAC2 protein, and identify the expression profile of HDAC2 mRNA in different tissues and testis of different stages of yak. Yaks were divided into juvenile(0.5-1 year), adult(4-5 years) and old groups(7-9 years).The samples of liver, kidney, lung, stomach, spleen, brain, heart and ovary from adult group and testis of three stages were collected. The yak HDAC2 gene was cloned by reverse transcription PCR (RT-PCR). The homology of HDAC2 gene sequence, as well as the amino acid sequence and structure of HDAC2 protein were predicated by bioinformatics. Real-time PCR was applied to identify the mRNA expression profile of HDAC2 in different tissues of yak. RT-PCR was applied to detect the mRNA expression of HDAC2 in yak testis of different stages. Chromogenic in situ hybridization (CISH) was applied to analysis the localization of HDAC2 mRNA in adult yak testis. Result showed that the open reading frame (ORF) of yak HDAC2 contained 1 467 bases and encoded 488 amino acids. The sequence of yak HDAC2 was highly conserved in mammals. Structure prediction indicated that HDAC2 was a hydrophilic liposoluble protein with a histone deacetylase domain. The mRNA of HDAC2 was highly expressed in ovary and testis. The expression of HDAC2 in juvenile stage was significantly higher than that in other stages. The mRNA of HDAC2 localized in multiple kinds of testicular cells except spermatid. The sequence of yak HDAC2 gene was cloned and the spatiotemporal expression pattern of this gene in testis was verified. This study provides some experimental data for further study on the role of HDAC2 in testicular development and spermatogenesis of yak.

Key words: Yak, Histone deacetylation, HDAC2, Gene cloning, Testis

CLC Number:

Q78
S823

YIN Shi, WANG Bin, QUNI Lamu, YANG Liuqing, YUAN Yujie, LI Jian. Cloning of Yak HDAC2 Gene and Its Expression Pattern in Testis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 222-229. doi: 10.7668/hbnxb.20191525.

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http://www.hbnxb.net/EN/Y2021/V36/I3/222

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