Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis Li, Guo & Zhang, 2017(Gastropoda：Succineidae)

doi:10.7668/hbnxb.20191638

Abstract

Abstract: In order to obtain the complete sequence of the mitochondrial genome of Oxyloma wujiaquensis Li, Guo & Zhang, 2017, analyze its composition characteristics, and explore the phylogenetic relationship between it and related groups in the family Succineidae and class Gastropoda, using Illumina Hiseq 2000 high-throughput and Sanger sequencing technology, MITOS online annotation and Genious R11.0 software, sequenced and analyzed the mitochondrial genome sequence of Oxyloma wujiaquensis. The results showed that the entire mitochondrial genome sequence was 14 086 bp (GenBank accession number:MT670402) with 75.64% AT and 24.36% GC, containing 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and non-coding regions.13 protein-coding genes used ATN, TTN as start codons and TAN, CAT and T as stop codons. In 22 tRNA genes, tRNA^Ser1 and tRNA^Ser2 lacked DHU arm. The A+T enrichment region was 42 bp, located between COX1 and tRNA^Val. The gene sequences and structures of mitochondrial genome of Oxyloma wujiaquensis and Succinea putris Linneaus, 1758 which belonged to the same family Succineidae were same basically. The mitochondrial genome sequence information of some species of Pulmonata and Opisthobranchia, construct a phylogenetic tree based on the mitochondrial genome sequence, and conduct phylogenetic analysis on the species of Succineidae. The results showed that these two species were gathered together, showing a close relationship. At present, there is very little information about the mitochondrial genome sequence of Succineidae in GenBank. This study will enrich the gene sequence information of Succineidae species and provide future research on the phylogenetic analysis, resource protection and genetic diversity of Succineidae provide basic data.

Key words: Oxyloma wujiqaquensis Li, Guo & Zhang, 2017, High-throughput sequencing, Mitochondrial genome, Sequence analysis

CLC Number:

AERZUGULI Maimaitituerxun, ZHANG Zijun, ZHANG Weihong. Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis Li, Guo & Zhang, 2017(Gastropoda：Succineidae)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 230-238. doi: 10.7668/hbnxb.20191638.

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References

[1] Pfeiffer L. Descriptions of ten new species of Bulimus,from the collection of H.Cuming,Esq[J]. Proceedings of the Zoological Society of London,1858,26(1):256-258.doi:10.1111/j.1469-7998.1858.tb06372.x.
[2] Roβmäβler,Kobelt E A. Iconographie der Landund Süsswasser-Mollusken,mit vorzüglicher Berück sichtigung dereuropäischen noch nicht abgebildeten Arten[M]. Dresden,Arnold:Band I,Heft I,1835:92.
[3] 李成有,郭阳阳,张卫红. 中国尖缘螺属一新种记述(柄眼目,琥珀螺科)[J]. 海洋科学,2017,41(11):130-133.
Li C Y,Guo Y Y,Zhang W H. A new species of Oxyloma from Xinjiang (Stylommatophora:Succineidae)[J]. Marine Sciences,2017,41(11):130-132.
[4] Boore J L. Animal mitochondrial genomes[J]. Nucleic Acids Research,1999,27(8):1767-1780.doi:10.1093/nar/27.8.1767.
[5] 杨婧,黄原. 线粒体基因组的高通量测序策略[J]. 生命科学,2016,28(1):112-117.doi:10.13376/j.cbls/2016015.
Yang J,Huang Y. Strategy of high-throughput sequencing technology in the mitochondrial genome sequencing[J]. Chinese Bulletin of Life Sciences,2016,28(1):112-117.
[6] Wang P,Yang H F,Zhou W C,Hwang C C,Zhang W H,Qian Z X. The mitochondrial genome of the land snail Camaena cicatricosa (Müller,1774) (Stylommatophora,Camaenidae):the first complete sequence in the family Camaenidae[J]. Zookeys,2014(451):33-48.doi:10.3897/zookeys.451.8537.
[7] Groenenberg D S J,Harl J,Duijm E,Gittenberger E. The complete mitogenome of Orcula dolium (Draparnaud,1801);ultra-deep sequencing from a single long-range PCR using the Ion-Torrent PGM[J]. Hereditas,2017,154(1):7.doi:10.1186/s41065-017-0028-2.
[8] Grande G,Templado J,Zardoya R. Evolution of gastropod mitochondrial genome arrangements[J]. BMC Evolutionary Biology,2008,8(1):61.doi:10.1186/1471-2148-8-61.
[9] Groenenberg D S,Pirovano W,Gittenberger E,Schilthuizen M. The complete mitogenome of Cylindrus obtusus (Helicidae,Ariantinae) using Illumina next generation sequencing[J]. BMC Genomics,2012,13(1):114.doi:10.1186/1471-2164-13-114.
[10] White T R,Conrad M M,Tseng R,Balayan S,Golding R,de Frias Martins A M,Dayrat B A. Ten new complete mitochondrial genomes of pulmonates (Mollusca:Gastropoda) and their impact on phylogenetic relationships[J]. BMC Evolutionary Biology,2011,11(1):295.doi:10.1186/1471-2148-11-295.
[11] 姜志磊,王大铭,王柏凤,宋新元. 双斑萤叶甲基因组调研及线粒体基因组分析[J].环境昆虫学报,2019,41(6):1287-1296.doi:10.3969/j.issn.1674-0858.2019.06.18.
Jiang Z L,Wang D M,Wang B F,Song X Y. Genome survey and mitochondrial genome analysis of Monolepta hieroglyphica[J]. Journal of Environmental Entomology,2019,41(6):1287-1296.
[12] 刘菲. 贺氏东栖螽线粒体基因组序列分析(直翅目:螽斯科)[J]. 基因组学与应用生物学,2017,36(8):3194-3199.doi:10.13417/j.gab.036.003194.
Liu F. Mitogenome sequence analysis of Xizicus howardi (Orthoptera:Tettigoniidae)[J]. Genomics and Applied Biology,2017,36(8):3194-3199.
[13] 张冬杰,何鑫淼,王文涛,汪亮,刘娣. 民猪全基因组序列测定与分析[J]. 东北农业大学学报,2018,49(11):9-17.doi:10.19720/j.cnki.issn.1005-9369.2018.11.0002.
Zhang D J,He X M,Wang W T,Wang L,Liu D. Determination and analysis of the whole genome sequence of Min pig[J]. Journal of Northeast Agricultural University,2018,49(11):9-17.
[14] Bernt M,Donath A,Jühling F,Externbrink F,Florentz C,Fritzsch G,Pütz J,Middendorf M,Stadler P F. MITOS:Improved de novo metazoan mitochondrial genome annotation[J]. Molecular Phylogenetics and Evolution,2013,69(2):313-319.doi:10.1016/j.ympev.2012.08.023.
[15] Perna N T,Kocher T D. Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes[J]. Journal of Molecular Evolution,1995,41(3):353-358.doi:10.1007/BF00186547.
[16] 钟健,刘增虎,杨伟克,朱峰,董占鹏.琥珀蚕线粒体全基因组测序及序列分析[J]. 昆虫学报,2017,60(8):936-949.doi:10. 16380/j. kcxb. 2017. 08. 011.
Zhong J,Liu Z H,Yang W K,Zhu F,Dong Z P. Sequencing and analysis of the complete mitochondrial genome of Antheraea assama (Lepidoptera:Saturniidae)[J]. Acta Entomologica Sinica,2017,60(8):936-949.
[17] 孙丽冉,齐迎菊,田晓轩. 哈氏蜈蚣线粒体基因组全序列分析[J]. 天津中医药,2018,35(3):225-229.doi:10.11656/j.issn.1672-1519.2018.03.19.
Sun L R,Qi Y J,Tian X X. Analysis of mitochondrial genome of Scolopendra subspinipes dehaani[J]. Tianjin Journal of Traditional Chinese Medicine,2018,35(3):225-229.
[18] 张玉波,史娅琴,孙翠英.长尾管蚜蝇Eristalis tenax (Linnaeus,1758)线粒体基因组测序及分析[J].基因组学与应用生物学,2019,38(12):5371-5378.doi:10.13417/j.gab.038.005371.
Zhang Y B,Shi Y Q,Sun C Y. Sequencing and analysis of the mitochondrial genome of Eristalis tenax (Linnaeus,1758)[J]. Genomics and Applied Biology,2019,38(12):5371-5378.
[19] Taanman J W. The mitochondrial genome:structure,transcription,translation and replication[J]. Biochim Biophys Acta,1999,1410(2):103-123.doi:10.1016/S0005-2728(98)00161-3.
[20] 李爱玲,张志芳,潘沈元.昆虫线粒体DNA A+T富集区的研究及其在分子进化中的应用[J].中国蚕业,2004,25(1):70-72.doi:10.3969/j.issn.1007-0982.2004.01.036.
Li A L,Zhang Z F,Pan S Y. Study on A+T enrichment region of insect mitochondrial DNA and its application in molecular evolution[J]. China Sericulture,2004,25(1):70-72.
[21] 李石柱,王艺秀,刘琴,吕山,王强,吴缨,张仪,周晓农.湖北钉螺线粒体基因组全序列测定研究[J].中国寄生虫学与寄生虫病杂志,2009,27(4):291-296.
Li S Z,Wang Y X,Liu Q,Lü S,Wang Q,Wu Y,Zhang Y,Zhou X N. Complete mitochondrial genome sequence of Oncomelania hupensis (Gastropoda:Rissooidea)[J]. Chin J Parasitol Parasit Dis,2009,27(4):291-296.
[22] Lin J H,Zhou W C,Ding H L,Wang P,Ai H M. The mitochondrial genome of the land snail Cernuella virgata (Da Costa,1778):the first complete sequence in the family Hygromiidae (Pulmonata,Stylommatophora)[J]. Zookeys,2016(589):55-69.doi:10.3897/zookeys.589.7637.
[23] 魏峦峦,沈斌,沈和定,陈诚,方磊,吴文健. 一种石磺科贝类线粒体基因组全序列分析[J].生物技术通报,2010(8):188-194,213.doi:10.13560/j.cnki.biotech.bull.1985.2010.08.006.
Wei L L,Shen B,Shen H D,Chen C,Fang L,Wu W J. Analysis of complete mitochondrial genome of platevindex mortoni of Onchidiidae (Mollusca:Gastropoda:Pulmonata)[J]. Biotechnology Bulletin,2010(8):188-194,213.
[24] Harrison R G. Animal mitochondrial DNA as a genetic marker in population and evolutionary biology[J]. Trends Ecol Evol,1989,4(1):6-11.doi:10.1016/0169-5347(89)90006-2.
[25] Moszczynska A,Locke S A,Mclaughlin J D,Marcogliese D J,Crease T J. Development of primers for the mitochondrial cytochrome c oxidase I gene in digenetic trematodes (Platyhelminthes) illustrates the challenge of barcoding parasitic helminths[J]. Molecular Ecology Resources,2009,9(S1):75-82.doi:10.1111/j.1755-0998.2009.02634.x.
[26] Hu M,Jex A R,Campbell B E,Gasser R B. Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing[J]. Nature Protocols,2007,2(10):2339-2344.doi:10.1038/nprot.2007.358.

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