Single Nucleotide Polymorphisms(SNPs)within Three Introns of Src Gene in Bos taurus

doi:10.7668/hbnxb.2010.02.019

Abstract

Abstract: The single nucleotide polymorphisms(SNPs)within the intron 6,8 and 9 of Src gene in Chinese Holstein cattle and Luxi Yellow cattle and Bohai Black cattle were investigated by nested-PCR,DNA sequencing technology,CRS-PCR(Created restriction site PCR)and PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism assay)method.Sequencing results showed that three SNPs were identified,which were 14062(C/T),17302(G/A)and 18107(T/C),respectively.In Chinese Holstein cattle and Luxi Yellow cattle,predominant allele were identical at 14062(C/T),17302(G/A)and 18107(T/C)site,which were C,G and C and the allelic frequencies were 0.671/0.647,0.583/0.640 and 0.558/0.577,respectively.However,the predominant allele of Bohai Black cattle at three SNPs site were T,G and C and the allelic frequencies were 0.525,0.913 and 0.763,respectively.Chi-square test indicated that the genotypic frequencies of 14062(C/T)site of Chinese Holstein cattle,Luxi Yellow cattle and Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium(P0.05),however,at 17302(G/A)site,the genotypic frequencies of all three population were out of the Hardy-Weinberg equilibrium(P0.05).The genotypic frequencies of 18107(T/C)locus in the Chinese Holsteins and Luxi Yellow cattle population are in Hardy-Weinberg equilibrium(P0.05),whereas being out of Hardy-Weinberg equilibrium for Bohai Black cattle(P0.05).The value of polymorphism information content indicated that three Chinese cattle breeds possessed intermediate genetic diversity for 14062(C/T),17302(G/A)and 18107(T/C)locus(0.25PIC0.5),except for Bohai Black cattle at locus 17302(G/A).

Key words: Bos taurus, Srcgene, Single nucleotide polymorphisms（SNPs）, Predominant allele

CLC Number:

S858.23

LIU Wen-yan, LI Qiu-ling, JU Zhi-hua, WANG Hong-mei, HUANG Jin-ming, LIU Shun-de, LI Rong-ling, ZHONG Ji-feng, WANG Chang-fa. Single Nucleotide Polymorphisms(SNPs)within Three Introns of Src Gene in Bos taurus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 99-105. doi: 10.7668/hbnxb.2010.02.019.

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