The Optimal Culture Conditions in vitro for Bovine Mammary Epithelial Cells

doi:10.7668/hbnxb.2010.S1.019

Abstract

Abstract: The objective of this study was to optimize the culture conditions in vitro for bovine mammary epithelial cells(BMECs). The medium,inoculation concentration,and the freeze preservation of BMECs were studied in culture condition by cell proliferation,which was evaluated by cell count trypan blue dying. The results show that the growth of cell cultured in Media Dulbecco's modified Eagle's medium(DMEM) was slower than in DMEM/F12,followed by F12 and then RPMI1640. The inoculation concentration of the epithelial cells indicated that 1 × 10⁴ cells status in each well was the best mode. The cell growth cycle experienced latent phase,logarithmic growth phase,stable phase and decline growth stages,and the growth curve accorded with the " S" -type growth law. The serum-freezing solution(90% FBS + 10% dimethyl sulfoxide) and medium-freezing solution(70% medium + 20% FBS +10% dimethyl sulfoxide) were used to preserve the bovine mammary epithelial cells. The cell proliferation and morphology of serum-freezing state were better than the medium-freezing state. The number of serum-freezing state was significant higher than medium state(P ＜ 0. 05) from the third day. The present study supplied some date for the method of culturing BMECs in vitro.

Key words: Bovine mammary epithelial cells, Culture in vitro, Growth curve, Medium, Inoculation concentration, Frozen preservation

CLC Number:

S858.23

LI Xi-yan, WANG Jia-qi, WEI Hong-yang, BU Deng-pan, HU Han, ZHOU Ling-yun. The Optimal Culture Conditions in vitro for Bovine Mammary Epithelial Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 79-85. doi: 10.7668/hbnxb.2010.S1.019.

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