Optimization of Induced and Cultivation System of Arbas Cashmere Goat iPSCs

doi:10.7668/hbnxb.2016.02.018

Abstract

Abstract: The induced pluripotent stem cells of Arbas cashmere goats (giPSCs) have significant values in genetic breeding, however, nowadays there are no compatible induced and cultivation medium systems of giPSCs.In order to establish a stable and complete induced and cultivation medium system, In this study, we showed that five different cultivation medium systems with different combination of serum and small molecules were able to generate the giPSCs.The growth status, subculture ability and reprogramming efficiency of giPSCs which derived from five different cultivation medium systems were observed, and pluripotency of these giPSCs was detected.At last, the influence of different combinations of small moleculars on the reprogramming efficiency and expression of pluripotent genes of giPSCs were analyzed.We found that serum promoted the generation of giPSCs and Vc, VPA and LiCL could accelerate the generation and self renewal of giPSCs.The research provided foundation for further study on stable and complete induced cultivation medium system of Arbas cashmere goat iPSCs, and experimental platform for embryonic stem cell line of Arbas cashmere goats.

Key words: Arbas cashmere goat, iPSCs, Induced and cultivation medium system, Small molecules

CLC Number:

S826

TAI Dapeng, Naimentana, NUO Mingtu, WANG Xiao, LIANG Hao, LIU Dongjun. Optimization of Induced and Cultivation System of Arbas Cashmere Goat iPSCs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 106-113. doi: 10.7668/hbnxb.2016.02.018.

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