Responses to Zn and EDTA Chelating Agents on Photosynthesis Characteristics of Alfalfa Leaves

doi:10.7668/hbnxb.2016.02.019

Abstract

Abstract: In order to explore the effective avenue on improving alfalfa(Medicage sativa L.) resistant ability under zinc stress, pot experiments were used to study the effect of EDTA on zinc accumulation, photosynthetic pigment content and chlorophyll fluorescence under zinc stress in different alfalfa leaves of three cultivars Medicago sativa ssp., Vitoria Alfalfa and Medicago sativa cv..The results of this study indicated that the zinc contents in three cultivar leaves were significantly higher than that in control group.Compared with zinc stress alone, the addition of EDTA strikingly enhanced the zinc absorption in leaves.In comparison with different cultivars, photosynthetic pigment content in Medicago sativa ssp.was higher than that in control group.After EDTA treatment, the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were 12.7%, 11.8%, 12.5%, 14.8% of Medicago sativa ssp.respectively, which were remarkably higher than that in the other two cultivars.Fo, Fv, Fm and Fv/Fm levels were increased by 5.4%, 14.9%, 18.1%, 4.0% of Medicago sativa ssp.with the treatment of EDTA.Our research indicated that EDTA was able to promote the accumulation of photosynthetic pigment, reduce the dissipation of excessive excitation energy, improve the transmission efficiency of photosynthetic electron, and minimize the damages to alfalfa leaves PSⅡ caused by zinc stress effectively, all of which coherently boosted resistance capability to zinc in Medicago sativa ssp.cultivar.As the results suggested, EDTA with the appropriate concentration at 10 μg could coefficient alleviate the damage of Zinc stress to Medicago sativa ssp., reduce dissipation of excess excitation and improve photochemical electron transport efficiency and efficiently protect from PSⅡ damage, EDTA obviously promoted the Zinc resistance of Medicago sativa ssp.under Zinc stress.

Key words: Zinc, Ethylene diamine tetraacetic acid, Medicage sativa L., Chlorophyll, Chlorophyll fluorescence

CLC Number:

S551.01

DAI Huiping, ZHAO Hua, JIA Genliang, FENG Baili. Responses to Zn and EDTA Chelating Agents on Photosynthesis Characteristics of Alfalfa Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 114-119. doi: 10.7668/hbnxb.2016.02.019.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.02.019

http://www.hbnxb.net/EN/Y2016/V31/I2/114

References

[1] 孙宁骁,宋桂龙.紫花苜蓿对镉胁迫的生理响应及积累特性[J].草业科学,2015,32(4):581-585.
[2] Qiu Z B,Li J T,Zhang M M,et al.He-Ne laser pretreatment protects wheat seedlings against cadmium-induced oxidative stress[J].Ecotoxicology and Environmental Safety,2013,8:135-141.
[3] 刘大林,杨俊俏,刘兆明,等.镉、铅胁迫对草地早熟禾幼苗生理的影响[J].草业科学,2015,32(2):224-230.
[4] Dai H P,Shan C J,Zhao H,et al.The difference in antioxidant capacity of four alfalfa species in response to Zn[J].Ecotoxicology and Environmental Safety,2015,114:312-317.
[5] 王红新,郭绍义,胡锋,等.螯合剂对铅锌尾矿改良基质上蓖麻幼苗生长和铅锌积累的影响[J].土壤学报,2012,49(3):491-498.
[6] 汪楠楠,胡珊,吴丹,等.柠檬酸和EDTA对铜污染土壤环境中吊兰生长的影响[J].生态学报,2013,33(2):631-639.
[7] Li X N,Yang Y L,Jia L Y,et al.Zinc-induced oxidative damage,antioxidant enzyme response and proline metabolism in roots and leaves of wheat plants[J].Ecotoxicology and Environmental Safety,2013,89(1):150-157.
[8] Luo C L,Shen Z G,Li X D.Enhanced phytoextraction of Cu,Pb,Zn and Cd with EDTA and EDDS[J].Chemosphere,2005,59(1):1-11.
[9] Seth C S,Misra V,Singh R R,et al.EDTA-enhanced Lead phytoremediation in sunflower(Helianthus annuus L.)hydroponic culture[J].Plant and Soil,2011,347(1):231-242.
[10] 杨明琰,陈华,梁语燕,等.EDTA强化黑麦草对铅污染土壤的诱导修复效应[J].环境科学与技术,2014,37(9):19-22.
[11] 胡亚虎,魏树和,周启星,等.螯合剂在重金属污染土壤植物修复中的应用研究进展[J].农业环境科学学报,2010,29(11):2055-2063.
[12] 王红新,胡锋,许信旺,等.EDTA对铅锌尾矿改良土壤上玉米生长及铅锌累积特征的影响[J].生态学报,2011,31(23):7125-7132.
[13] 蒋先军,骆永明,赵其国,等.镉污染土壤植物修复的EDTA调控机理[J].土壤学报,2003,40(2):205-209.
[14] Liu J,Zhou Q X,Wang S.Evaluation of chemical enhancement on phytoremediation effect of Cd-Contaminated soils with Calendula officinalis L.[J].International Journal of Phytoremediation,2010,12(5):503-515.
[15] Cui S,Zhou Q X,Wei S H,et al.Effects of exogenous chelators on phytoavailability and toxicity of Pb in Zninnia elegans Jacq[J].Journal of Hazardous Materials,2007,146(1/2):341-346.
[16] 张敬锁,李花粉,衣纯真,等.有机酸对活化土壤中镉和小麦吸收镉的影响[J].土壤学报,1999,36(1):61-66.
[17] 孙健,铁柏清,秦普丰,等.EDTA调控下灯心草和龙须草对铅锌尾矿污染土壤的修复潜力[J].环境科学研究,2006,19(4):105-110.
[18] Dai H P,Wei Y,Yang T X,et al.Influence of photosynthesis and chlorophyll synthesis on Cd accumulation in populus×canescens[J].Journal of Food Agriculture & Environment,2012,10(1):1020-1023.
[19] Cambrolle J,Mancilla-Leyton J M,Munoz-Valles S,et al.Zinc tolerance and accumulation in the salt-marsh shrub Halimione portulacoides[J].Chemosphere,2012,86(9):867-874.
[20] 邱莉萍,张兴昌.Cu、Zn、Cd和EDTA对土壤酶活性影响的研究[J].农业环境科学学报,2006,25(1):30-33.
[21] 王学锋,罗晓东,皮运清.EDTA对Pb、Cd及Pb-Cd处理下茼蒿中叶绿素的影响[J].环境科学与技术,2011,34(1):52-55.
[22] 代惠萍,赵桦,曹婷,等.Zn²⁺胁迫对4种紫花苜蓿光合色素的影响[J].西北农业学报,2014,23(3):128-132.

Please choose a citation manager

Content to export