Cloning and Expression Analysis of CO4 Gene in the Different Light Qualities and Light Transfers of Grapevine

doi:10.7668/hbnxb.2018.04.013

Abstract

Abstract: To explore the effects of different light qualities on the expression of CO4 gene in grapevine plantlet in vitro. The full-length cDNA sequence of CO4 was cloned from Vitis riparia×V.labrusca Beta in vitro by RT-PCR. Bioinformatics analysis of the second level structure, and phylogenetic tree of protein. The result showed that the size of CO4 gene fragment was 663 bp, the open reading frame (ORF)of CO4 gene was 747 bp, including 248 amino acid coding regions. By use bioinformation analysis, CO4 of Beta was a hydrophobic, unstable protein with good lipid solubility. CO4 had a signal peptide, it can be inferred that it was a transmembrane protein. Phylogenetic tree indicated that CO4 of Beta (Vitis riparia×V.labrusca)had the highest evolutionary relationship with Eucalyptus grandis (Eucalyptus grandis Hill ex Maiden).qRT-PCR analysis showed that the CO4 expressed specifically and strongly in the treatment of red to blue light (control), which increased 5.39 fold in comparison to the control. The difference was not significant when exposed to the treatment of white to red light and red to white light. However the expression of CO4 was the lowest in the white to blue light treatment, which was 54% of the control. Fluorescence parameter analysis showed that the maximum photochemical efficiency (Fv/Fm), potential activity (Fv/Fo)and photochemical quenching coefficient (qP)were significantly higher than those of the control under red to blue light. The non photochemical quenching coefficient (NPQ)was the lowest under that treatment, but was the highest under red light treatment, it was the lowest under this treatment. The CO4 of Beta was more sensitive to light. The treatments of red to blue light could not only promote the expression of CO4, but also could improve the Beta Grapevine in vitro photosynthetic capacity. Thus, red to blue light played an important role in the process of morphogenesis of Beta Grapevine in vitro.

Key words: Grapevine, CO4, Gene expression, Light quality, Chlorophyll fluorescence parameters

CLC Number:

Q78
S663.03

FAN Qi, MA Yanni, CHEN Baihong, ZUO Cunwu, MAO Juan. Cloning and Expression Analysis of CO4 Gene in the Different Light Qualities and Light Transfers of Grapevine[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 90-97. doi: 10.7668/hbnxb.2018.04.013.

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