Effect of EBR Treatment on Fruit Browning and Related Enzyme Gene Expression in Sweet Cherry

doi:10.7668/hbnxb.20190386

Abstract

Abstract: The browning of sweet cherry fruit not only affects its nutritional value but also affects its commerciality.In order to study the relationship of browning and the expression level of PPO,POD,CAT,SOD genes under different EBR concentration treatment,sweet cherry were used as test materials.Changes of fruit browning index,PPO,POD,CAT,SOD activity and relative expression of PPO,POD,CAT and SOD genes were measured during storage.The results showed that the fruit browning index of the control group was significantly higher than that of the EBR treatment groups after 30 days of storage,and the fruit browning of the control group was more serious.The activity of PPO and POD in the control group were always higher than that in the EBR groups during the whole storage process.CAT and SOD activity in EBR treated fruits were always higher than that in untreated fruits after storage for 15 days.The relative expressions of PPO and POD genes in the control group were significantly higher than those in the EBR treatment groups.The relative expressions of CAT and SOD genes in EBR treated fruits were significantly higher than those in untreated fruits after storage for 30 days.The above results showed that EBR treatment could inhibit the relative expression of PPO and POD genes and reduce the activity of PPO and POD.It could promote the relative expression of CAT and SOD genes and improved the activity of CAT and SOD.The effect of 5 μmol/L EBR treatment was better,which could maintain the quality and flavor of sweet cherry fruit.

Key words: Sweet cherry, 2,4-epibrassinolide, Browning, Enzyme activity, Gene expression

CLC Number:

Q78
S662.03

ZHOU Hui, WANG Yunxiang, WANG Yu, LI Wensheng, CHANG Hong, ZHOU Jiahua, WANG Baogang. Effect of EBR Treatment on Fruit Browning and Related Enzyme Gene Expression in Sweet Cherry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 24-30. doi: 10.7668/hbnxb.20190386.

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