Cloning and Expression Analysis of Drought-Related Gene CsMYB94 in Cucumber

doi:10.7668/hbnxb.20190941

Abstract

Abstract: Drought stress is one of the most serious threats to cucumber quality and yield. In order to better understand the molecular mechanism of cucumber's response to water stress,this experiment obtained a differentially expressed gene CsaV3_3G039670 (log₂FC=1.10) related to drought and significantly up-regulated expression from the previous work. Its homologous gene AtMYB94 was passed in Arabidopsis regulating the epidermal wax content of the plant to improve drought resistance,it was named CsMYB94. The CDS sequence of CsMYB94 gene was 885 bp in length and encoded 294 amino acids. By analyzing the protein domain,the CDS sequence contained two DNA-binding domains,which were R2R3 type MYB transcription factors. Protein analysis found that the molecular formula of CsMYB94 protein was C₁₄₂₆H₂₂₄₂N₄₀₄O₄₅₄S₁₁; the expected molecular weight was 3.27 ku; the theoretical isoelectric point was 6.53; it was a hydrophobic protein and an unstable protein. Analysis by subcellular localization software revealed that CsMYB94 protein was localized in the nucleus. The phylogenetic tree analysis showed that CsMYB94 in cucumber had the closest relationship with MYB94 in white algae and closer to MYB94 in pumpkin,bitter gourd and melon. The results of qRT-PCR analysis showed that CsMYB94 had the highest expression in cucumber leaves,but relatively low expression in male flowers,tendrils and stems. Therefore,it is speculated that CsMYB94 also responds to drought stress by regulating the wax content of cucumber,and the specific regulation path needs further study.

Key words: Cucumber, CsMYB94, Drought response, Expression analysis

CLC Number:

S642.2
X172

GONG Siyu, CHEN Haiyan, GUO Dongxue, YU Yue, WU Tao. Cloning and Expression Analysis of Drought-Related Gene CsMYB94 in Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 18-23. doi: 10.7668/hbnxb.20190941.

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