Effect of Acid Treatment on Expression of Genes Related to Tuber Formation of Potato

doi:10.7668/hbnxb.20191215

Abstract

Abstract: The roots of potato plants were treated with phosphoric acid solution (pH 4) for 4 d,then four stages of stolons were collected as samples, which were before stolon tip hook (stage Ⅰ), stolon tip hook (stage Ⅱ), swelling tips (stage Ⅲ) and initial tuber formation (stage Ⅳ). The samples were used to determine the expression levels of genes related to tuber formation. The 45-day-old plants treated with acid solution formed the maximum stolon at stage Ⅱ, and produced the largest quantity of tubers which were 53.2% and 74.5% higher than that of the 55 and 60-day-old plants. The expressions levels of StHd3a, StFD, StSUSY4, StHXK, StPIN1, StABA and StSP6A of acid treatment group were obviously higher than those of control group in all stages. Moreover, at the stolon development in acid treatment group of stage Ⅰ and stage Ⅱ, compared with the control group, the StGA expressions were significantly increased by 49.4% and 25.1%,the StAGL8 expressions were significantly increased by 119.7% and 140.1%, while the StAGPase expressions were significantly reduced by 60.1% and 41.7%, and the StSSS expressions were significantly reduced by 23.2% and 21.6%. However, the StCOL expressions in acid treated plants were not significantly different from that of the control grup. In conclusion, the acid treatment could affect the complex process of potato tuber formation through the regulation of the above tested genes (except StCOL) expression levels or patterns during the tuberization.

Key words: Potato, Acid treatment, Stolon development stage, Gene expression

CLC Number:

GUO Jinting, GAO Yuliang, ZHANG Yan, LI Kuihua. Effect of Acid Treatment on Expression of Genes Related to Tuber Formation of Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 47-54. doi: 10.7668/hbnxb.20191215.

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