Cloning and Expression Analysis of GsTIFY6B Associated with Saline and Alkali Stress in Glycine soja

doi:10.7668/hbnxb.2018.04.012

Abstract

Abstract: In order to determine the molecular characterization and expression patterns of TIFY family genes involved in saline and alkali stress in Glycine soja, according to previous transcriptome data, GsTIFY6B was obtained by homologous cloning using cDNA of Glycine soja G07256, with extremely tolerant ability to saline and alkali stress, as its template. The full-length CDS was 1 047 bp. GsTIFY6B protein was an unstable protein with 348 amino acids. Its molecular weight was 36.8 ku and its isoelectric point was 9.12. Phylogenetic tree analysis showed the closest homology of GsTIFY6B to the TIFY family proteins of GmTIFY6B, VrTIFY6B, CcTIFY6B and AdTIFY6A. All those proteins had the conserved domains TIFY and Jas. The transcription level of GsTIFY6B in different parts of Glycine soja as well as response to saline and alkali stress and hormone treatment was detected by Real-time fluorescence quantitative PCR. The results showed that the highest relative expression level of GsTIFY6B in roots. Under salt stress, the expression of GsTIFY6B in roots and leaves showed an up-regulation.Under alkali stress, the expression of GsTIFY6B in roots and leaves showed the first down-regulation and then up-regulation expression. It is speculated that the gene might be involved in the defense reaction of saline-alkali stress.Under the ABA treatment, GsTIFY6B showed down-regulated expression in the roots and up-regulated expression at 6, 12 h in leaves. Under MeJA stress, GsTIFY6B expression was decreased first and then increased in roots, but showed up-regulation in leaves. This study suggested that GsTIFY6B could positively respond to saline and alkali stress by participating in the ABA and MeJA signaling pathways. These results will provide a theoretical basis for the later study on the molecular mechanism of saline-alkali-tolerant response of Glycine soja.

Key words: Glycine soja, GsTIFY6B, Gene cloning, Saline and alkali stress

CLC Number:

Q785
S565.03

YAN Wenfei, CHENG Fansheng, JIANG Xinqiang, LIU Cuixia, ZHU Dan. Cloning and Expression Analysis of GsTIFY6B Associated with Saline and Alkali Stress in Glycine soja[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 82-89. doi: 10.7668/hbnxb.2018.04.012.

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