Abstract: The fatty acid composition is one of the important goals to improve rapeseed quality, different environment and cultivation conditions can altered fatty acid composition.This experiment was to promote the improvement of rapeseed fatty acid composition from molecular aspects. Brassica napus 420 was used as material, the design method of ternary quadratic regression orthogonal combination was adopted, chlorophyll content and fad2 and fad3 gene were studied in different periods and different cultivation conditions. And found that:the content of chlorophyll increased first and then decreased with the increased of nitrogen and boron content, but decreased with the increased of density; the changes of the expressions of fad2 and fad3 were similar to those of chlorophyll,besides, it found that there was positive correlation between the amount of chlorophyll content and fad2 and fad3 gene expression by linear regression analysis. In addition, we found that the expression of fad2 and fad3 was higher in A2B1C2 and A2B1C3, but had lower expression in A1B3C1, A3B3C1 and A1B3C3, that may contribute to the accumulation of other fatty acids (such as oleic acid, etc.) at lower expression, so as to improve the quality of rapeseed is of great significance,it needs to be further studied.

Key words: Brassica napus, Chlorophyll, Different cultivation conditions, Fatty acid synthesis, Gene expression

CLC Number: 

• S565.03

• Q78