Effects of Nitrogen Application Rate in Drip Irrigation on Chlorophyll Fluorescence Characteristics and Carbon Metabolism of Grape in Desert Area

doi:10.7668/hbnxb.20190344

Abstract

Abstract: In order to study the effects of nitrogen application rate on the photosynthetic characteristics and assimilation product accumulation of Cabernet gernischet grapes at different growth stages, four urea application rates under drip irrigation conditions were set:150(N1), 300(N2), 450 (N3), 600 kg/ha(N4), 0 kg/ha was set to control(CK), the effects of different nitrogen nutrition on photosynthetic fluorescence parameters and carbon metabolism related indexes of grape leaves at growth period (5 days before flowering,20 days after flowering,50 days after flowering) were analyzed. The results showed that appropriate nitrogen application improved the chlorophyll content and RuBp carboxylase activity of leaves, enhanced the photosynthesis of vines, and increased the chlorophyll fluorescence of leaves which represented as the increase of effective photochemical efficiency (Fv'/Fm'), photochemical quenching coefficient (qP) and actual photochemical efficiency of PSⅡ(ΦPSⅡ) and the decrease of non-photochemical quenching coefficient (NPQ) in leaves. Compared with CK, Fv'/Fm', ΦPSⅡ, and qP under N2 treatment in DAF20 and DAF50 were significantly increased by 16.10%, 17.80%, and 21.49%, 21.13%, and 11.77%, 19.73%, respectively.Appropriate nitrogen application increased the activity of sucrose metabolism-related enzymes and soluble sugar content, but decreased starch content.And throughout the growth period, SPS, SS, and sucrose had the highest values under N3, glucose and fructose had the highest values under N2, while NI and AI had the highest values under N2, N2 and N1 respectively under DBF5, DAF20 and DAF50. Compared with CK, N2 treatment increased the content of soluble sugar, tannin, anthocyanin and the titratable acid content, promoted the fruit setting rate and the ratio of sugar to acid with yield of 14.50 t/ha.In conclusion, the application range of urea from 300 to 450 kg/ha enhanced the photosynthesis of leaves, promoted the synthesis of soluble sugars, thus providing sufficient nutrition for the growth and development of grape fruits.

Key words: Grape, Nitrogen, Chlorophyll fluorescence, Carbon metabolism, Yield quality

CLC Number:

S66
S143.1

HAN Lina, MA Zonghuan, WANG Ying, HU Zijing, SHI Xingyun, MAO Juan, CHEN Baihong. Effects of Nitrogen Application Rate in Drip Irrigation on Chlorophyll Fluorescence Characteristics and Carbon Metabolism of Grape in Desert Area[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 170-177. doi: 10.7668/hbnxb.20190344.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20190344

http://www.hbnxb.net/EN/Y2020/V35/I2/170

References

[1] 杨夕. 水分胁迫下施加硝态氮对葡萄幼苗氮素代谢及相关酶的影响[D]. 石河子:石河子大学, 2018.
Yang X. Effects of nitrate nitrogen on nitrogen metabolism and related enzymes in grape seedlings under water stress[D].Shihezi:Shihezi University, 2018.
[2] 赵阳, 刘怀锋, 赵庆祥, 郑小能, 牛媛, 马富裕. 设施条件不同灌量对弗雷无核葡萄成熟期光合生理特征及品质形成的影响[J]. 新疆农垦科技, 2018, 41(5):11-15. doi:10.3969/j.issn.1001-361X.2018.05.012.
Zhao Y, Liu H F, Zhao Q X, Zheng X N, Niu Y, Ma F Y. Effects of different irrigation conditions on photosynthetic physiological characteristics and quality formation of frey seedless grapes during maturity[J]. Xinjiang Agricultural Reclamation Science and Technology, 2018, 41(5):11-15.
[3] Pascual M,Villar J M,Rufat J. Water use efficiency in peach trees over a four-years experiment on the effects of irrigation and nitrogen application[J]. Agricultural Water Management, 2016, 164(2):253-266. doi:10.1016/j.agwat.2015.10.021.
[4] 陈昱辛, 贾悦, 崔宁博, 杨永刚, 赵璐, 胡笑涛, 龚道枝. 滴灌水肥一体化对柑橘叶片光合生长、产量及水分利用效率的影响[J]. 灌溉排水学报, 2018(S2):50-58. doi:10.13522/j.cnki.ggps.20180521.
Chen Y X, Jia Y, Cui N B, Yang Y G, Zhao L, Hu X T, Gong D Z. Effects of integrated management of water and fertilizer on citrus photosynthesis, yield and water use efficiency[J]. Journal of Irrigation and Drainage, 2018(S2):50-58.
[5] 李洪艳. 土壤水分对葡萄植株生长发育的影响[D].上海:上海交通大学, 2009.
Li H Y. Effects of soil moisture on the growth and development of grape plants[D]. Shanghai:Shanghai Jiaotong University, 2009.
[6] 杨首乐, 邓忠, 翟国亮, 李迎, 宋志伟, 景明. 干旱区水氮耦合效应对棉花生长性状及产量的影响[J].中国农学通报, 2016, 32(24):103-108.
Yang S L, Deng Z, Zhai G L, Li Y, Song Z W, Jing M. Effects of water and nitrogen coupling on cotton growth characteristics and yield in arid area[J]. Chinese Agricultural Science Bulletin, 2016, 32(24):103-108.
[7] 李艳红, 石德杨, 许玉良, 李凡华, 王奎良. 水肥一体化技术在鲜食葡萄上的应用[J]. 落叶果树, 2019, 51(2):51-53. doi:10.13855/j.cnki.lygs.2019.02.017.
Li Y H, Shi D Y, Xu Y L, Li F H, Wang K L. Application of water and fertilizer integration technology in fresh grape[J]. Deciduous Fruit Trees, 2019, 51(2):51-53.
[8] 李红燕. 不同补光措施、调亏灌溉及有机肥对酿酒葡萄生长及品质的影响[D]. 杨凌:西北农林科技大学, 2016.
Li H Y. Effects of different light-filling measures, regulated deficit irrigation and organic fertilizer on the growth and quality of wine grapes[D]. Yangling:Northwest A&F University, 2016.
[9] Njovu A M, Mahoo H F, Msogoya T J, Tiisekwa B M. Effect of irrigation regimes on yield and quality of grapes (Vitis vinifera L.) cv.. Makutopora red' in Dodoma tanzania[J]. Journal of Biology, Agriculture and Healthcare, 2017, 7(4):87-99.
[10] Pankaj B. Yield, fruit quality and water productivity of drip fertigated assam lemon (Citrus limon)[J]. International Journal of Agricultural Engineering, 2013, 6(2):339-344.
[11] Panigrahi P, Srivastava A K. Water and nutrient management effects on water use and yield of drip irrigated citrus in vertisol under a sub-humid region[J]. Journal of Integrative Agriculture, 2017, 16(5):1184-1194. doi:10.1016/S2095-3119(16)61500-9.
[12] 施明, 朱英, 司海丽, 王锐, 孙权, 王振平. 灌溉对贺兰山东麓酿酒葡萄生长、产量及品质的影响[J]. 节水灌溉, 2013(10):1-3. doi:10.3969/j.issn.1007-4929.2013.10.001.
Shi M, Zhu Y, Si H L, Wang R, Sun Q, Wang Z P. Effect of irrigation on growth, yield and quality of wine grape in Eastern Foot of Helan Mountain[J]. Water Saving Irrigation, 2013(10):1-3.
[13] Hebbar S S, Ramachandrappa B K, Nanjappa H V, Prabhakar M. Studies on NPK drip fertigation in field grown tomato (Lycopersicon esculentum Mill.)[J]. European Journal of Agronomy, 2004, 21(1):117-127. doi:10.1016/S1161-0301(03)00091-1.
[14] 史星雲, 李强, 张军, 金娜, 王鑫, 王多文, 牟德生, 徐珊珊. 滴灌条件下水肥耦合对酿酒葡萄生长发育及果实品质的影响[J]. 西北农业学报, 2019,28(2):225-236. doi:10.7606/j.issn.1004-1389.2019.02.010.
Shi X Y, Li Q, Zhang J, Jin N, Wang X, Wang D W, Mou D S, Xu S S. Effects of water and fertilizer coupling on growth and quality of wine grape under drip irrigation[J]. Acta Agriculture Boreali-occidentalis Sinica, 2019, 28(2):225-236.
[15] 曾庆华, 徐峰, 郑文才, 周凯, 周前艳. 不同施氮量对北玫葡萄品质和经济效益的影响[J]. 耕作与栽培, 2018(3):21-22,30. doi:10.13605/j.cnki.52-1065/s.2018.03.007.
Zeng Q H, Xu F, Zheng W C, Zhou K, Zhou Q Y. Effects of different nitrogen application rates on quality and economic benefits of Beimei grape[J]. Tillage and Cultivation, 2018(3):21-22, 30.
[16] 胡紫璟. 不同氮素水平对酿酒葡萄'蛇龙珠'植株碳氮代谢影响的研究[D]. 兰州:甘肃农业大学, 2016.
Hu Z J. The study on the influence of different nitrogen levels on metabolism of carbon and nitrogen in wine grape Cabernet Gernischet tree[D]. Lanzhou:Gansu Agricultural University, 2016.
[17] 马宗桓. 滴灌条件下不同施氮时期对酿造葡萄碳氮代谢的影响[D]. 兰州:甘肃农业大学, 2016.
Ma Z H. Effects of different nitrogen application stages on carbon and nitrogen metabolism of brewed grape under drip irrigation[D]. Lanzhou:Gansu Agricultural University, 2016.
[18] 任凌燕, 陈楠, 徐刚. 降糖袋泡茶总多糖的含量测定研究[J]. 福建茶叶, 2019, 41(4):1-3. doi:10.3969/j.issn.1005-2291.2019.04.001.
Ren L Y, Chen N, Xu G. Determination of total polysaccharide in hypoglycemic teabags[J]. Tea in Fujian, 2019, 41(4):1-3.
[19] 邓榆川, 刘卫国, 袁小琴, 袁晋, 邹俊林, 杜俊波, 杨文钰. 套作大豆苗期茎秆纤维素合成代谢与抗倒性的关系[J]. 应用生态学报, 2016, 27(2):469-476. doi:10.13287/j.1001-9332.201602.024.
Deng Y C, Liu W G, Yuan X Q, Yuan J, Zou J L, Du J B, Yang W Y. Relationship between cellulose synthesis metabolism and lodging resistance in intercropping soybean at seedling stage[J]. Chinese Journal of Applied Ecology, 2016, 27(2):469-476.
[20] 张倩, 辛力, 亓雪龙, 孙玉刚, 孙山, 张静. 肉桂精油对甜樱桃果实品质和货架期的影响[J]. 核农学报, 2015, 29(9):1737-1742. doi:10.11869/j.issn.100-8551.2015.09.1737.
Zhang Q, Xin L, Qi X L, Sun Y G, Sun S, Zhang J. Effects of cassai oil on the shelf-life and quality of sweet cherry fruits[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(9):1737-1742.
[21] 张彪. 不同砧穗组合对戈壁区酿酒葡萄生长和品质的影响[D]. 兰州:甘肃农业大学, 2014.
Zhang B. Effects of different rootstock combinations on the growth and quality of wine grapes in Gobi region[D]. Lanzhou:Gansu Agricultural University, 2014.
[22] 李耕, 高辉远, 刘鹏, 杨吉顺, 董树亭, 张吉旺, 王敬锋. 氮素对玉米灌浆期叶片光合性能的影响[J]. 植物营养与肥料学报, 2010, 16(3):536-542. doi:10.11674/zwyf.2010.0304.
Li G, Gao H Y, Liu P, Yang J S, Dong S T, Zhang J W, Wang J F.Effects of nitrogen fertilization on photosynthetic performance in maize leaf at grain filling stage[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(3):536-542.
[23] 马冬云, 郭天财, 宋晓, 王晨阳, 韩巧霞, 岳艳军, 查菲娜. 施氮对冬小麦旗叶RuBp羧化酶活性及叶绿素荧光参数的影响[J]. 西北植物学报, 2010, 30(11):2197-2202.
Ma D Y, Guo T C, Song X, Wang C Y, Han Q X, Yue Y J, Zha F N. Effects of nitrogen fertilizer application on RuBp carboxylase activity and chlorophyll fluorescence parameters in flag leaves of winter wheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(11):2197-2202.
[24] 张旺锋, 勾玲, 王振林, 李少昆, 余松烈, 曹连莆. 氮肥对新疆高产棉花叶片叶绿素荧光动力学参数的影响[J]. 中国农业科学, 2003, 36(8):893-898. doi:10.3321/j.issn:0578-1752.2003.08.006.
Zhang W F, Gou L, Wang Z L, Li S K, Yu S L, Cao L P. Effect of nitrogen on chlorophyll fluorescence of leaves of High-yielding cotton in Xinjiang[J]. Scientia Agricultura Sinica, 2003, 36(8):893-898.
[25] 尹海龙, 田长彦. 氮调控对盐环境下甜菜功能叶光系统Ⅱ荧光特性的影响[J]. 植物生态学报, 2013, 37(2):122-131. doi:10.3724/SP.J.1258.2013.00013.
Yi H L, Tian C Y. Effects of nitrogen regulation on photosystem Ⅱ chlorophyll fluorescence characteristics of functional leaves in sugar beet (Beta vulgaris) under salt environment[J]. Chinese Journal of Plant Ecology, 2013, 37(2):122-131.
[26] Schaffer A A, Liu K C, Goldschmidt E E, Boyer C D, Goren R. Citrus leaf chlorosis induced by sink removal:starch, nitrogen, and chloroplast ultrastructure[J]. Journal of Plant Physiology, 1986, 124(1-2):111-121. doi:10.1016/S0176-1617(86)80183-3.
[27] 温志静, 郭延平, 张雯, 毛海燕, 梁俊. 叶喷不同水平氮肥对苹果果实淀粉和糖及代谢相关酶活性的影响[J]. 西北农业学报, 2018, 27(6):846-853. doi:10.7606/j.issn.1004-1389.2018.06.011.
Wen Z J, Guo Y P, Zhang W, Mao H Y, Liang J. Effects of spraying different levels of nitrogen fertilizer on starch and sugar and metabolic enzyme activities in apple fruit[J]. Journal of Northwest Agricultural Sciences, 2018, 27(6):846-853.
[28] 陈洋, 赵宏伟. 氮素用量对春玉米穗位叶蔗糖合成关键酶活性的影响[J]. 玉米科学, 2008, 16(1):115-118. doi:10.13597/j.cnki.maize.science.2008.01.034.
Chen Y, Zhao H W. Effect of nitrogen application on activities of key enzymes of sucrose synthesis in the leaf located near the ear of spring maize[J]. Journal of Maize Sciences, 2008, 16(1):115-118.
[29] 李丹. 甜菜蔗糖代谢相关酶活性与糖积累关系的研究[D]. 哈尔滨:东北农业大学, 2009.
Li D. Study on the relationship between sucrose metabolism related enzyme activities and sugar accumulation in sugar beet[D]. Harbin:Northeast Agricultural University, 2009.
[30] 孙虎, 王月福, 李尚霞, 王成霞, 王铭伦. 施氮量对不同类型花生蔗糖合成及产量的影响[J]. 植物营养与肥料学报, 2008, 14(2):398-402. doi:10.11674/zwyf.2008.0231.
Sun H, Wang Y F, Li S X, Wang C X, Wang M L. Effect of nitrogen levels on yield and sucrose synthesis of leaves in different types of peanut[J]. Journal of Plant Nutrition and Fertilizers, 2008, 14(2):398-402.
[31] 克热木·伊力, 新居直祐. 不同氮素施用量对葡萄叶、枝、根碳水化合物含量的影响[J]. 新疆农业大学学报, 2001, 24(1):64-68. doi:10.3969/j.issn.1007-8614.2001.01.013.
Karim A, Naosuka N. Effects of different amount of nitrogen fertilizer on carbohydrate content in leaves, shoots and roots of grape[J]. Journal of Xinjiang Agricultural University, 2001, 24(1):64-68.
[32] 彭福田, 张青, 姜远茂, 彭勇, 周鹏. 不同施氮处理草莓氮素吸收分配及产量差异的研究[J]. 植物营养与肥料学报, 2006, 12(3):400-405. doi:10.3321/j.issn:1008-505X.2006.03.019.
Peng F T, Zhang Q, Jiang Y M, Peng Y, Zhou P. Effect of nitrogen application on nitrogen absorption, distribution and yield of strawberry[J]. Journal of Plant Nutrition and Fertilizers, 2006, 12(3):400-405.

Please choose a citation manager

Content to export