Transcriptome Analysis of Quinoa Seedlings in Response to Low Nitrogen

doi:10.7668/hbnxb.20194769

Abstract

Abstract:

In order to explore the molecular mechanism of low nitrogen response in quinoa, low nitrogen response genes were screened to reveal the adaptive changes in quinoa response to low nitrogen.Based on the seedling growth observation and chlorophyll synthesis detection,we analyzed the transcriptome changes of quinoa after 5 d and 30 d under nitrogen deficiency conditions.The results showed that roots were preferentially developed under nitrogen starvation condition.Older leaves turned yellow or dropped down under both low nitrogen and nitrogen starvation conditions,therefore younger leaves could maintain green.Higher NUE was shown in both low and nitrogen starvation conditions.GO enrichment analysis indicated that significantly differential expressed genes were mainly involved in integral component of membrane,membrane,oxidation-reduction process,metabolic process,ATP binding,and metal ion binding.After 5 d of low or nitrogen starvation supply,KEGG enrichment analysis showed that phenylpropyl biosynthesis and glutathione metabolism were the most significant metabolic pathways compared with high nitrogen.After 30 d of treatment,the most significant metabolic pathway was the carbon metabolic pathway.The key genes in response to low nitrogen in quinoa were further explored.The results showed that peroxidase,glutathione S-transferase and ascorbate peroxidase genes were up-regulated and their expressions were higher after 5 days of low nitrogen and nitrogen deficiency treatment.The genes of phosphoglycerate kinase,cysteine synthetase,glyceraldehyde 3-phosphate dehydrogenase (NADP)and phosphoenolpyruvate carboxylase were up-regulated and the expression levels were higher after 30 days of low and low nitrogen treatment.The results of qRT-PCR agreed with the RNA-Seq.

Key words: Quinoa, Low nitrogen, Phenylpropanoid biosynthesis, Glutathione metabolism, Carbon metabolism

YANG Yashu, YU Peiyi, WANG Jianhua, SHAN Jianan, PEI Hongbin, YANG Liyan. Transcriptome Analysis of Quinoa Seedlings in Response to Low Nitrogen[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 143-153. doi: 10.7668/hbnxb.20194769.

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References 57

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基因号 Gene number	基因注释 Gene annotation	引物序列(5'—3') Primer sequence(5'—3')
CqEF-1a	—	F:GTACGCATGGGTGCTTGACAAACTC
		R:ATCAGCCTGGGAGGTACCAGTAAT
AUR62004699	硝酸还原酶	F:GCAGAGGAGGACATATTG
		R:TCTCAACAACATACCATACTT
AUR62029383	亚硝酸还原酶	F:ATAAGGATGATGTTGATGTTAGA
		R:TTGCTCACTCGTTGTTAC
AUR62041208	谷氨酰胺合成酶	F:CAAGCACCAGGAGAAGAC
		R:TGTTACCACCACGGAATG
AUR62042896	磷酸烯醇丙酮酸羧化酶	F:TGGTGGTGGATATGAAGA
		R:GACAGATTGAGTAGGATGAG
AUR62038255	MYB44	F:TGTAGTAGTAGCGAGAATA
		R:CTTGTTAAGGCATCATCT
AUR62043570	尿卟啉原脱羧酶	F:TGCCCTGAAACACCACTTG
		R:CAGTCCAGCCCAATCACATT

基因号 Gene number	基因注释 Gene annotation	引物序列(5'—3') Primer sequence(5'—3')
CqEF-1a	—	F:GTACGCATGGGTGCTTGACAAACTC
		R:ATCAGCCTGGGAGGTACCAGTAAT
AUR62004699	硝酸还原酶	F:GCAGAGGAGGACATATTG
		R:TCTCAACAACATACCATACTT
AUR62029383	亚硝酸还原酶	F:ATAAGGATGATGTTGATGTTAGA
		R:TTGCTCACTCGTTGTTAC
AUR62041208	谷氨酰胺合成酶	F:CAAGCACCAGGAGAAGAC
		R:TGTTACCACCACGGAATG
AUR62042896	磷酸烯醇丙酮酸羧化酶	F:TGGTGGTGGATATGAAGA
		R:GACAGATTGAGTAGGATGAG
AUR62038255	MYB44	F:TGTAGTAGTAGCGAGAATA
		R:CTTGTTAAGGCATCATCT
AUR62043570	尿卟啉原脱羧酶	F:TGCCCTGAAACACCACTTG
		R:CAGTCCAGCCCAATCACATT

处理 Treatment	总生物量/g Total biomass	地上部干质量/g Aboveground dry weight	地下部干质量/g Underground dry weight	根冠比 Root-shoot ratio	氮素利用效率 NUE
HN	1.77±0.11ab	1.47±0.12b	0.30±0.02a	0.20±0.08a	2.80±0.13c
LN	1.96±0.12a	1.72±0.13a	0.24±0.02b	0.14±0.01c	4.38±0.12a
NS	0.98±0.11c	0.83±0.07c	0.15±0.01c	0.18±0.01ab	3.08±0.14b

处理 Treatment	总生物量/g Total biomass	地上部干质量/g Aboveground dry weight	地下部干质量/g Underground dry weight	根冠比 Root-shoot ratio	氮素利用效率 NUE
HN	1.77±0.11ab	1.47±0.12b	0.30±0.02a	0.20±0.08a	2.80±0.13c
LN	1.96±0.12a	1.72±0.13a	0.24±0.02b	0.14±0.01c	4.38±0.12a
NS	0.98±0.11c	0.83±0.07c	0.15±0.01c	0.18±0.01ab	3.08±0.14b

样品 Samples	全部Reads数/条 Total Reads	质控后Reads数/条 Clean Reads	质控后Bases数/bp Clean Bases	Q30碱基数占比/% Q30	GC含量/% GC content
HN5d	43 666 970	21 833 485	6 515 802 112	94.06	44.13
LN5d	39 524 672	24 305 980	7 250 637 130	93.90	43.96
NS5d	48 611 960	19 762 336	5 897 857 974	93.90	43.99
HN30d	49 542 228	24 771 114	7 382 666 816	93.64	43.83
LN30d	55 391 736	22 149 363	6 599 239 224	94.02	44.03
NS30d	44 298 726	27 695 868	8 247 014 636	94.13	44.19

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