Molecular Cloning and Spatio-temporal Expression Analysis of KLF6 in Goat

doi:10.7668/hbnxb.20191543

Abstract

Abstract: This study was performed to obtain the sequence of Kruppel-like factor 6(Kruppel-like factor 6, KLF6) CDS, clarify its expression pattern in tissues and subcutaneous adipocyte of goats. The KLF6 gene sequence of goat was cloned by RT-PCR, and the sequence of KLF6 gene was analyzed by biological software and online website. Quantitative real-time PCR (qPCR) was used to detect the expression abundance of KLF6 in various goat tissues and the expression level of subcutaneous adipocytes at different stages of lipogenesis differentiation. The results showed that the full-length sequence of goat KLF6 gene was 1 233 bp, including 957 bp of CDS region, which encoded 318 amino acid residues, and closest relative was the cattle and the sheep. KLF6 gene was widely expressed in various the investigated tissues of goats, the expression level of KLF6 was higher in adipose tissue, which was significantly higher than that in other tissues(P<0.01). The expression of KLF6 in goat subcutaneous adipocytes induced for 60 h was significantly higher than that in preadipocytes (P<0.01). The sequence of goat KLF6 gene was obtained and its molecular characteristics were identified. It was found that it was highly expressed in adipose tissue, and the expression level after differentiation was significantly higher than that before differentiation.It provides important data for revealing the regulation of KLF6 on the differentiation of goat adipocytes.

Key words: Goat, KLF6, Tissue expression, Preadipocytes, Cell differentiation

CLC Number:

Q78
S826

WANG Jianglin, WANG Yong, MENG Qingyong, ZHU Jiangjiang, LIN Yaqiu. Molecular Cloning and Spatio-temporal Expression Analysis of KLF6 in Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 226-232. doi: 10.7668/hbnxb.20191543.

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