Analysis of EGLN1 Gene Expression, Mutation Characteristics and Adaptability in Tibetan Sheep

doi:10.7668/hbnxb.201751045

Abstract

Abstract: In order to reveal the adaptation mechanism of plateau hypoxia in Tibetan sheep, the EGLN1 gene was selected as the research object, and the genetic variation characteristics of the gene in sheep population were analyzed for molecular breeding. The quantitative PCR and PCR-SSCP techniques were used to detect the EGLN1 gene expression in different tissues and the nucleotide variation in intron6-intron7 regions of sheep. The results showed that the EGLN1 gene was expressed in the tissues of the two sheep populations, and there was a difference in tissues. Among them, the expression of Tibetan sheep was lower than Hu sheep, and it was higher in heart, liver and kidney. Six SNPs, one insertion and one deletion were detected. There was a statistically significant difference in alleles and genotype frequencies between the two groups (P<0.05). The frequency of dominant allele B and the predominant genotypes AB were significantly higher in Tibetan sheep than that in Hu sheep (P<0.01). The exon and intron regions of EGLN1 gene in Tibetan sheep had high genetic variation, rich polymorphism and great potential for selection. It can be used as a candidate gene for Tibetan sheep to adapt the high altitude hypoxia. The results will provide the basic data for genetic improvement and germplasm innovation in Tibetan sheep.

Key words: Tibetan sheep, EGLN1 gene, Gene expression, Mutation characteristics, Adaptability

CLC Number:

S826.03
Q78

LIU Xiu, ZHANG Yaqiang, LI Shaobin, WANG Jiqing, HU Jiang, SHA Yuzhu, ZHANG Wei, LUO Yuzhu. Analysis of EGLN1 Gene Expression, Mutation Characteristics and Adaptability in Tibetan Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 226-231. doi: 10.7668/hbnxb.201751045.

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