Bioinformatics Analysis and Tissue Expression of Chicken Toll-like Receptor 15

doi:10.7668/hbnxb.201750964

Abstract

Abstract: Toll-like receptor that involve innate immunity is one kind of model molecule, and it can recognize a wide range of pathogen-associated molecular patterns and has important effects on anti-infectious immunity. The TLR15 gene was cloned from the Hy-Line brown chicken by RT-PCR, the extracellular region of TLR15 gene was expressed in prokaryotic cells.The fusion protein was detected by SDS-PAGE and Western Blot assay, and were used to immunize Kunming-mice to prepare polyclonal antibodies. Then the expression of chTLR15 in the main organs was detected by immunohistochemical method. The results showed that the open reading frame of Hy-Line brown chicken TLR15 gene was composed of 2 607 bp in length encoding 868 amino acids, the extracellular region was 1 962 bp, the molecular weight of recombinant protein was regarding 72 ku, and the fusion protein was used for producing the antiserum in mice with the antibody titer 1:10 000. TLR15 protein was mainly expressed in the spleen, but less in liver and lung. The study would provide basic materials for the molecular study on poultry TLR15, and construct a basis for the development of anti-infectious biological agents.

Key words: Hy-Line brown chicken, Toll-like receptor15(TLR15), Expression, Sequence analysis

CLC Number:

Q78
S831

XING Bojian, YU Miao, WU Di, XU Xiao, LI Lianyan, LIU Yufen. Bioinformatics Analysis and Tissue Expression of Chicken Toll-like Receptor 15[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 232-238. doi: 10.7668/hbnxb.201750964.

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