Abstract:

Research on the expression pattern and function of FaesANT gene in long-homostyle(LH)common buckwheat and uncovering its roles involved in floral organ development will help to provide gene resources for the molecular breeding and germplasm innovation of buckwheat.The full-length FaesANT gene was isolated from long-homostyle common buckwheat by combining PacBio SMRT(Single Molecule Real-Time)sequencing and RACE technique.FaesANT expression levels in different tissues and floral buds at sequential developmental stages of long-homostyle common buckwheat were separately detected by using paraffin sections and qRT-PCR.Moreover,the FaesANT gene function involved in floral development was investigated by using virus-induced gene silencing(VIGS)in long-homostyle common buckwheat.The results were as follows,FaesANT cDNA(1 982 bp)comprised a 1 632 bp ORF encoding a 543 amino acid transcription factor.Phylogenetic tree analysis grouped the FaesANT into the eudicots ANT lineage.FaesANT expression levels were mainly detected in stamen,pistil and young fruit of long-homostyle common buckwheat and showed no significant differences among them.However,only weak transcripts were detected in the stems,leaves and tepals.During floral bud differentiation,obvious expression of FaesANT gene was detected when pistil and stamen primordia formation,and FaesANT expression level reached peak during filament elongation stage of stamen.Then,FaesANT expression decreased gradually with the floral bud development and dropped to the lowest level after flowering.TRV2-FaesANT-treated long-homostyle plants with strong phenotypic changes produced flowers with style-and filament-length decreased,and stamen number reduced.In addition,some TRV2-FaesANT-treated long-homostyle plants displayed part stamens with anther homeotically converted into tepaloid structure.Moreover,FaesANT expression decreased significantly in the flowers of FaesANT-silenced long-homostyle plants with strong phenotypic changes.It suggested that FaesANT was mainly involved in regulating filament and style length,and determining stamen number of common buckwheat based on the gene expression pattern and phenotypes of VIGS-treated long-homostyle common buckwheat flowers.

Key words: Fagopyrum esculentum, ANT homologous gene, Gene expression, Gene silence, Long homostyle

CLC Number: 

• S517荞麦