Resource Screening and Germplasm Creation of Brassica napus Resistant to Clubroot

doi:10.7668/hbnxb.201751077

Abstract

Abstract: In order to screen the cruciferous germplasms resistant to clubroot, the resistant genes were transferred to Brassica napus. A collection of 30 cruciferae accessions was screened using Plasmodiophora brassicae pathotype 4 in Chengdu, and the significance analysis, European clustering analysis and correlation analysis of incidence and disease index were carried out. According to the classification of disease index, seven of the accessions demonstrated high resistance(HR), including three Chinese cabbages, three turnips and one cabbage. There were two medium sensitivity (MS) materials, two susceptible (S) materials and 19 high sensitivity (HS) materials. Among them, the high sensitivity materials were rapeseed. The average incidence of these 30 materials was 69.13%, and the average incidence index was 51.45. The results showed that there was a significant difference between the disease index. The European clustering analysis was consistent with the disease index analysis, and there was a linear correlation between the morbidity and the disease index. One resistant germplasm of turnip, Debra, was used to cross with the core parent of Brassica napus susceptible to clubroot, and the cytology identification and molecular marker-assisted selection were used to determine F₁ hybrid authenticity. The results showed that the three F₁ hybrids obtained were dark green, with thick leaves, less bristles on the leaf margins, and elliptical leaves. The overall traits were between the two parents and favored the female parent. No symptoms were observed after inoculation. 15 pairs of SSR primers with good amplifying polymorphisms were screened to screen the three hybrids and their parents, which presented both the parental bands. It was clear that the three F₁ hybrids were all true hybrids. The 7 high resistance materials and 3 true hybrids could provide the material basis for further studying the resistance mechanism of clubroot and breeding new rapeseed varieties resistant to clubroot.

Key words: Clubroot, Germplasm screening, Resistance of cruciferae, Distant hybridization, Identification of hybrid authenticity, Brassica napus

CLC Number:

LIANG Fenghao, LANG Lina, LIU Yaping, WANG Jing, ZHANG He, XU Aixia, HUANG Zhen. Resource Screening and Germplasm Creation of Brassica napus Resistant to Clubroot[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 219-225. doi: 10.7668/hbnxb.201751077.

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