The Physiological Mechanism of the Increase of the Infection of the Maize Resistance to the Fusarium graminearum

doi:10.7668/hbnxb.201750779

Abstract

Abstract: To clear the effect and mechanism of brassinosteroid (BR) on enhancing the maize resistance against the Fusarium graminearum infection, maize Zhengdan 958 was used as the experimental material, two treatments were spraying BR and unspraying BR on maize plants,10 days later after treatment processing, inoculated Fusarium graminearum spore on the stem,and then investigated and compared the maize incidence after treatment of spraying BR, determined the exact effect of BR on improving the maize resistance against the Fusarium graminearum infection. The results showed that under the natural condition without spraying BR, after inoculating Fusarium graminearum spore on maize stem, there were obvious traces of pathogen infection and spread along the inoculation needle on the stem surface, the medullary tissue was seriously dark brown, and almost all the nodes had been infected with pathogens. But the invasion area by the pathogen in intramedullary tissue was significantly reduced of the maize stem sprayed BR 10 days before inoculating Fusarium graminearum spore, and the morbidity was significantly reduced, this indicated that BR enhanced the ability of maize to resist the infection. The growth and development of Fusarium graminearum colony were not affected obviously when cultured in the PDA medium containing BR. After BR treatment, the content of proline in maize leaves increased, Real-time PCR analyzed the expression of gene ZmP5CS which encoded with proline synthase, and found that its expression was indeed induced by BR. At the same time, the content of soluble sugar in corn increased extremely significant, and the enzyme activity of protective enzyme SOD and PAL significantly increased and POD increased extremcly significant, it was shown that BR improved the resistance of maize to Fusarium graminearum infestation by raising the corn's own immunity. The conclusion was that BR treatment had no obvious effect on the growth and development of Fusarium graminearum colony, the reason for the increase of the maize resistance against the Fusarium graminearum infection was not the inhibition of BR on the growth and development of Fusarium graminearum, but by raising corn proline synthesis, protective enzyme activity, soluble sugar content and improve corn immune ability.

Key words: Brassinosteroid, Fusarium graminearum, Physiological mechanism, Maize

CLC Number:

S432
S513.01

ZHANG Hao, FENG Jiajia, SUN Dandan, YAN Qingdi, ZHAO Yalin, WANG Shan, WANG Pingping, ZHAO Yazhuo, WANG Qian, WANG Fengru, DONG Jingao. The Physiological Mechanism of the Increase of the Infection of the Maize Resistance to the Fusarium graminearum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 213-218. doi: 10.7668/hbnxb.201750779.

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