Acta Agriculturae Boreali-Sinica ›› 2025, Vol. 40 ›› Issue (3): 215-224. doi: 10.7668/hbnxb.20195708

Special Issue: Plant protection Animal husbandry

• Animal Husbandry·Fisheries·Veterinarian • Previous Articles     Next Articles

Effect of Polygonum cuspidatum Aqueous Extract on Porcine epidemic diarrhoea virus Replication in IPEC-J2 Cells

WANG Yuxuan1,2, YANG Zhi1,2, LIU Haoyang1,2, WANG Xinghai1,2, YANG Fulin1,2, LU Hongde1,2, HE Zhiyuan1,2, , DONG Hong1,2,   

  1. 1 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China
    2 Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China
  • Received:2024-12-22 Published:2025-07-04

Abstract:

The aim of this study was to explore the reproductive adaptability of PEDV in porcine intestinal epithelial cells(IPEC-J2)and to evaluate the inhibitory effect of Polygonum cuspidatum water extract on it in vitro.The effects of different trypsin concentrations and different time on the activity of IPEC-J2 cells and the expression of PEDV N protein in cells were evaluated by cytotoxicity assay and Western Blot assay.The virus titer,PEDV N gene transcription level and N protein expression were detected by TCID50,Real-time Fluorescence Quantitative PCR and Western Blot.The effect of water extract of P.cuspidatum on the proliferation activity of IPEC-J2 cells was detected by CCK-8 method.Further,the inhibitory effect of P.cuspidatum water extract on the expression of PEDV N gene and N protein was detected by Real-time Fluorescence Quantitative PCR and Western Blot experiments,and the effect of P.cuspidatum water extract on each stage of PEDV life cycle was explored.The results showed that when the concentration of trypsin was 2 μg/mL,trypsin had no effect on the cells.At this time,PEDV showed the best reproductive adaptability and reached the highest virulence value after 36 h.When the concentration of P.cuspidatum water extract was lower than 100 μg/mL,there was no cytotoxicity at 12,24 h.The water extract of P.cuspidatum significantly inhibited the expression of N gene under Co-treatment and Post-treatment,and significantly inhibited the expression of PEDV N gene mRNA and N protein in a dose-dependent manner.The water extract of P.cuspidatum had a significant inhibitory effect on PEDV N gene mainly in the replication and proliferation stage of the virus life cycle,and had no significant inhibitory effect in other stages.In summary,2 μg/mL trypsin had the best adaptability to PEDV reproduction,and the water extract of P.cuspidatum had a significant inhibitory effect on PEDV in vitro,which mainly acted on the replication and value-added stage of PEDV life cycle.

Key words: Polygonum cuspidatum water extract, PEDV, IPEC-J2, Adaptability, Inhibition in vitro

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Cite this article

WANG Yuxuan, YANG Zhi, LIU Haoyang, WANG Xinghai, YANG Fulin, LU Hongde, HE Zhiyuan, DONG Hong. Effect of Polygonum cuspidatum Aqueous Extract on Porcine epidemic diarrhoea virus Replication in IPEC-J2 Cells[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 215-224. doi: 10.7668/hbnxb.20195708.

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