Screening the Immune Enhancer of Porcine epidemic diarrhea virus Subunit Vaccine

doi:10.7668/hbnxb.2016.02.033

Abstract

Abstract: This study successfully constructed pQZ-COE-LTB expression vector and expressed the soluble recombinant protein, which was chosen for PEDV subunit vaccine candidate.In order to enhance the immunogenicity of the PEDV subunit vaccine, we selected CVC1302 and CVC1303 as immune enhancer, combined with PEDV subunit vaccine.Immunize 4-week-old ICR mice, second immunize conducted after 14 d, then collected the 14, 21, 28, 55 d mouse serum after first immune.Agar diffusion test and ELISA kits of detection of serum IgG antibody levels, intestinal tissue sIgA levels were conducted.The results showed that PEDV subunit vaccine combined with CVC1303 immune enhancer group produced significantly higher levels of IgG and sIgA antibodies than CV777 inactivated vaccine positive control group, indicating CVC1303 immune enhancer can effectively enhance the immunogenicity of the PEDV subunit vaccine.

Key words: PEDV, COE, Immune enhancer, Immunogenicity

CLC Number:

S858.28

CHEN Jin, HUANG Chunjuan, YU Xiaoming, HOU Liting, QIAO Xuwen, ZHENG Qisheng, HOU Jibo. Screening the Immune Enhancer of Porcine epidemic diarrhea virus Subunit Vaccine[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 205-210. doi: 10.7668/hbnxb.2016.02.033.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.02.033

http://www.hbnxb.net/EN/Y2016/V31/I2/205

References

[1] 朱迪国,宋建德,袁丽萍,等.2013-2014年全球猪流行性腹泻重大疫情分析[J].中国动物检疫,2014,31(10):42-46.
[2] Chen Q,Li G,Stasko J,et al.Isolation and characterization of Porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States[J].Journal of Clinical Microbiology,2014,52(1):234-243.
[3] Temeeyasen G,Srijangwad A,Tripipat T,et al.Genetic diversity of ORF3 and spike genes of Porcine epidemic diarrhea virus in Thailand[J].Infection,Genetics and Evolution:Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases,2014,21:205-213.
[4] 李龙.2011年冬哺乳仔猪病毒性腹泻最新流行情况[J].养猪,2012,21(2):96-96.
[5] 张志,董雅琴,刘爽,等.我国部分省份猪流行性腹泻的流行病学监测[J].中国动物检疫,2014,31(10):47-51.
[6] 郑逢梅,霍金耀,赵军,等.2010-2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析[J].病毒学报,2013,29(2):197-205.
[7] Nguyen-Xuan Huy,Sae-Hae Kim,Moon-Sik Yang,et al.Immunogenicity of a neutralizing epitope from Porcine epidemic diarrhea virus:M cell targeting ligand fusion protein expressedin transgenic rice calli[J].Plant Cell Rep,2012,31(10):1933-1942.
[8] 杨榕,顾超逸,李郁,魏建忠等.猪流行性腹泻病毒COE基因的原核表达和免疫原性分析[J].安徽农业大学学报,2013,40(6):955-958.
[9] 满坤,杨兵,覃湘婕,等.猪流行性腹泻病毒中和抗原表位基因的原核表达、纯化及免疫原性鉴定[J].黑龙江畜牧兽医,2013,11:26-29.
[10] 向敏,张洁,高其双,等.猪流行性腹泻病毒COE核酸疫苗的构建及免疫原性[J].中国兽医学报,2013,33(11):1627-1630.
[11] 李鹏,王家乡,陈碧军.仔猪大肠杆菌病K88-K99-987P-F41四价亚单位疫苗的研制Ⅳ[J].疫苗生产与免疫效力试验,2006,27(9):55-58.
[12] Rosales-Mendoza S,Soria-Guerra R E,De Jesús Olivera-Flores M T,et al.Expression of Escherichia coli heat-labile enterotoxin b subunit(LTB)in carrot(Daucus carota L.)[J].Plant Cell Reports,2007,26(7):969-976.
[13] 郭鹰,邹全明,朱永红,等.Balb/c小鼠口服幽门螺杆菌疫苗rLTB-HspA的免疫应答[J].免疫学杂志,2004,20(3):201-203,207.
[14] 陈明,李超,王瑞,等.大肠杆菌不耐热肠毒素B亚单位在毕赤酵母中的表达及鉴定[J].西南农业学报2010,23(6):2093-2097.
[15] Ricci S,Medaglini D,Rush C M,et al.Immunogenicity of the B monomer of Escherichia coli heat-labile toxin expressed on the surface of Streptococcus gordonii[J].Infect Immun 2000;68(2):760-766.
[16] 霍军,宋予震,董青.猪流行性腹泻病毒流行毒株COE基因的原核表达及免疫原性分析[J].江苏农业科学,2014,42(9):34-36.
[17] 李龙.2011年冬哺乳仔猪病毒性腹泻最新流行情况[J].养猪,2012,21(2):96.
[18] Park S,Kim H,Song D,et al.Molecular characterization and phylogenetic analysis of Porcine epidemic diarrhea virus(PEDV)field isolates in Korea[J].Archives of Virology,2011,156(4):577-585.
[19] 焦茂兴,吴锋,刘德辉,等.猪流行性腹泻病毒重组腺病毒疫苗的构建及小鼠免疫试验[J].中国畜牧兽医,2012,39(2):11-16.
[20] Tae-Jin Kang,So-Chon Han,Moon-Sik Yang,et al.Expression of synthetic neutralizing epitope of Porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants[J].Protein Expression and Purification,2006,46(1):16-22.
[21] Tae-Jin Kang,Young-Sook Kim,Yong-Suk Jang,et al.Expression of the synthetic neutralizing epitope gene of Porcine epidemic diarrhea virus in tobacco plants without nicotine[J].Vaccine,2005,23(17-18):2294-2297.

Please choose a citation manager

Content to export