Detection of Viruses Infecting Cucurbit Vegetables in Shandong and Identification of Subgroup of the Cucumber mosaic virus Isolates

doi:10.7668/hbnxb.2016.02.034

Abstract

Abstract: In order to detect and identify the viral causing agent that infecting cucurbit vegetable plants in Shandong, 867 cucurbit plant samples of suspected viral diseases from 11 vegetable planting areas in Shandong were collected during July to August, 2014.9 primer pairs specific to Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Watermelon mosaic virus (WMV), Tobacco mosaic virus (TMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Melon yellow spot virus (MYSV), Cucurbit chlorotic yellows virus (CCYV), Prunus necrotic ringspot ilarvirus (PNRSV) and Melon necrotic spot virus (MNSV) were used respectively to perform PCR detection.The results showed that viruses carried rate was 34.8%, 10.4%, 20.0%, 41.7%, 27.0%, 7.8%, 2.6%, 1.7%, 0 and 0.9% respectively.The natural infection of these viral diseases except PNRSV in Shandong was confirmed, and the incidence of CMV and TMV were higher than others.Two or more than two viruses mixed infections were also very common in field.At the same time, those CMV positive samples from 11 regions were selected for subgroup classification.Coat protein(CP) gene and the whole RNA3 nucleotide sequences were determined.Sequence alignment and phylogenetic analyses were performed.The results showed that the CMV isolates of cucurbit vegetables in Shandong area were all members of CMV subgroup IB with the highest similarity to a South Korea isolate As(AF013291), no other strains were found.

Key words: Cucurbit viral disease, Cucumber mosaic virus, RT-PCR, Subgroup

CLC Number:

S432.4+1

SUN Xiaohui, WANG Shusen, GAO Lili, QIAO Ning, LIU Yongguang, ZHAO Jing, ZHU Xiaoping. Detection of Viruses Infecting Cucurbit Vegetables in Shandong and Identification of Subgroup of the Cucumber mosaic virus Isolates[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 211-217. doi: 10.7668/hbnxb.2016.02.034.

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