The Analysis of Gansu Alpine Merino and Hu Sheep HIF-2α Gene Genetic Characteristics and Adaptability to High Altitude Hypoxia

doi:10.7668/hbnxb.2016.03.013

Abstract

Abstract: To study the relationship between the variations of HIF-2a gene and adaption to plateau in sheep,we detected the 540 sheep including 200 Gansu Alpin Merino and 340 Hu sheep,and used the PCR-SSCP to analyze the SNPs of part of the eleventh exon and intron of HIF-2a gene.The results showed that 6 SNPs corresponding to 4 alleles(A,B,C and D) and 10 kinds of genotypes(AA,BB,CC,DD,AB,AC,BC,AD,BD and CD) were detected in HIF-2a gene in 2 sheep breed.The 5 SNPs existed in the eleventh exon and the one SNPs laid in the eleventh intron and the genotype DD was not detected in Gansu Alpine Merino.The advantaged genotype AB with frequency of 0.345 0 and 0.164 7 was detected in both sheep breed.The HIF-2a gene has abundant polymorphism in 2 sheep breed and the frequency of alleles has significantly difference in two popul ations(P<0.01).As the selecting of environment with plateau and low oxygen,the HIF-2a gene was selected and clustered in plateu sheep.The sheep with the genetype AA,BB and AB can adapt in plateau environment more better and the adaptive capacity of other sheep with genetype CD,BD and DD was poor.

Key words: HIF-2α gene, Gansu Alpine Merino, Adaptability, Polymorphism

CLC Number:

Q78
S826

DANG Pengju, LI Shaobin, WANG Jiqing, LIU Xiu, HONG Wei, HU Jiang, LUO Yuzhu. The Analysis of Gansu Alpine Merino and Hu Sheep HIF-2α Gene Genetic Characteristics and Adaptability to High Altitude Hypoxia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 88-93. doi: 10.7668/hbnxb.2016.03.013.

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