Abstract: To elucidate the polymorphism of SLA-DRA gene coding region and the protein structural and functional characteristics in Yantat black pig,the PCR-SSCP and cloning sequencing were used to analyze the polymorphism of SLA-DRA gene coding region,and many bioinformatics methods were used to predict and analyze the physicochemical characteristics,protein structure,structural domain,and functions.Results showed that a total of 11 SNPs were identified in SLA-DRA gene exon 1,exon 2 and exon 4, which made 7 amino acids changed,no variation in exon 3 and exon 4 had the richest polymorphism.There were 759 bp nucleotides of the SLA-DRA gene coding region,which encoded 252 amino acids and one terminator.The protein of SLA-DRA gene was a kind of acidity unstable,and hydrophilic protein,whose secondary structure was the mixed and contained the large of random coil.The putative SLA-DRA gene protein included of 2 immunoglobulin domains,2 N-glycosylation sites and 10 phosphorylation sites.Function analysis showed that the high odds predicted from signal transduction,receptor,stress response,immune response and growth factor in SLA-DRA gene protein.This study may provide many theory information for candidate genes selection in pig disease resistance molecular breeding.

Key words: SLA-DRA gene, Yantai black pig, Coding region, Polymorphism, Protein structure, Molecular evolutionary tree

CLC Number: 

• S828

• Q78