Abstract: In order to analyze sequence variations of KAP11-1 gene,single nucleotide polymorphisms (SNPs) of Oula sheep,Gansu Alpine Merino,Tan sheep,Hu sheep and Qinghai merino sheep (a total of 749 individuals) were detected using PCR-SSCP and DNA sequencing techniques in this study.Population genetics characteristics such as allele frequency,genetic heterozygosity,effective number of alleles and polymorphism information content (PIC) were analyzed simultaneously.The results suggested that four SNPs (c.93C/T,c.240G/A,c.285C/G/A and c.331A/G) in KAP11-1 gene of 5 sheep populations were discovered,and c.331A/G was non-synonymous substitution.Allele Aexhibited the highest frequency (87.70%) among five sheep populations,and it was the most common allele,allele B was the next common with the allele frequency of 11.03%,the frequency of allele C and D were the lowest,which were 0.53% and 0.74% respectively.Population genetics results showed that heterozygosity,effective number of alleles and polymorphic information content of Tan sheep were higher than those of another four sheep populations. KAP11-1 gene is one of the major candidate genes of wool economic traits.The nucleotide sequence variations of KAP11-1 gene will lead to amino acid changes of KAP11-1,then eventually affect wool fibre structure and wool economic traits.

Key words: Sheep, KAP11-1 gene, Nucleotide sequence variations, PCR-SSCP, Single nucleotide polymorphism

CLC Number: 

• Q78

• S826