The Analysis of Glutenin,Gliadin and Agronomic Traits for Nine Purple and Blue Grain Wheat

doi:10.7668/hbnxb.2016.05.016

Abstract

Abstract: In order to understand the background and utility value for purple or blue grain wheat,the glutenin and gliadin of 9 purple or blue grain wheat are analyzed by the method of polyacrylamide gel electrophoresis (PAGE).The high molecular weight glutenin subunits (HMW-GS) results from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that there were 8 types of subunits and 6 types of subunit combination in 9 materials.Subunit 1 (66.7%),7+8 (55.6%) and 5+10 (55.6%) were the majority subunit in Glu-A1, Glu-B1 and Glu-D1 loci,respectively.Subunit combination 1/7+8/5+10 and Null/7+8/5+10 were the mainly type in materials.In low molecular weight glutenin subunits (LMW-GS),there are 7 (41.2%) bands showing in all materials which isolating totally 17 bands.The gliadin results from acid polyacrylamide gel electrophoresis (A-PAGE) showed that there are 7 (35.0%) bands showing in all materials which isolating totally 20 bands.The clustering analysis based on the bands from LMW-GS and gliadin indicated that the genetic distance of the materials is from 0.07 to 0.37.These 9 materials can be divided into four categories at the level of 0.22.Materials HR-1 and NH-1 can be gathered at the value of 0.07.Purple grain wheat Luozhen 1,HR-1 and NH-1 can be used in breeding and genetics programs for variety improvement because of its good agronomic traits and quality subunits 1 and 5+10.

Key words: Wheat, Purple grain, Blue grain, Glutenin, Gliadin, Agronomic trait

CLC Number:

S512.03
Q78

CHENG Xueni, LI Yiming, LEI Zhongping, WU Jun, YANG Qunhui, CHEN Xinhong, ZHAO Jixin. The Analysis of Glutenin,Gliadin and Agronomic Traits for Nine Purple and Blue Grain Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 108-113. doi: 10.7668/hbnxb.2016.05.016.

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References

[1] 杨峰,孙玉,鲁晋秀,等.黑小麦研究现状及优质高产栽培技术[J].陕西农业科学,2014(11):109-112.
[2] 孙群,孙宝启,王建华.黑粒小麦子粒色素的遗传研究[J].种子,2003(4):33-34,37.
[3] 汤开东,周生伟,白建军,等.黑粒小麦籽粒颜色的遗传分析[J].农业科学与技术:英文版,2011,12(8):1141-1143.
[4] 李楠楠,张卫东,高庆荣,等.小麦山农066559紫色性状的遗传分析[J].麦类作物学报,2013,33(1):18-22.
[5] 兰素缺,李杏普,刘玉平.蓝粒小麦籽粒色素遗传研究[J].华北农学报,2008,23(z1):12-14.
[6] 宗学凤,张建奎,李帮秀,等.小麦籽粒颜色与抗氧化作用[J].作物学报,2006,32(2):237-242.
[7] 裴自友,李爱霞,庄丽芳,等.黑粒小麦漯珍1号的C-分带及GISH和PAGE分析[J].麦类作物学报,2005,25(4):15-18.
[8] 刘惠芳,张名位,池建伟,等.黑色食品新资源河东乌麦营养成分的评价[J].中国粮油学报,1999,2(2):3-5.
[9] 孙玉.优质黑粒小麦76的营养价值与开发利用[J].中国食物与营养,1999,1(1):42-43.
[10] Shewry P R,Tatham A S,Forde J,et al.The classification and nomenclature of wheat gluten protein:a reassessment[J].Journal of Cereal Science,1986,4(86):97-106.
[11] Payne P I,Lawrence G J.Catalogue of alleles for the complex gene loci,Glu-A1,Glu-B1,Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat[J].Cereal Research Communication,1983,11:29-35.
[12] Zillman R R,Bushuk W.Wheat cultivar identification by gliadin electrophoregrams.3.catalogue of electrophoregram formulas of Canadian wheat cultivars[J].Canadian Journal of Plant Science,1979,59(2):287-298.
[13] 王瑞,张改生,Zeller F J,等.小麦资源胚乳蛋白Glu-1、Glu-3、Gli-1基因位点变异特点[J].作物学报,2006,32(4):625-629.
[14] 程雪妮,赵继新,乔森涛,等.陕西关中地区部分小麦新品种(系)的HMW-GS组成分析[J].麦类作物学报,2008,28(6):983-987.
[15] Gupta R B,Macritchie F.A rapid one-step one-dimensional SDS-PAGE procedure for analysis of subunit composition of glutenin in wheat[J].Journal of Cereal Science,1991,14:105-109.
[16] Draper S R.ISTA variety committee report of the working group for biochemical tests for cultivar identification 1983-1986[J].Seed Science and Technology,1987,15:431-434.
[17] 赵继新,武军,陈新宏,等.几种小麦族亲缘植物麦谷蛋白和醇溶蛋白研究[J].河北农业大学学报,2008,31(6):1-6.
[18] Nei M.Analysis of gene diversity in subdivided population[J].Proceedings of the National Academy of Sciences,1974,70(12):3321-3323.
[19] 刘春雷,杨雪,张丽琴,等.722份河南省小麦新品系高分子量谷蛋白亚基多样性分析[J].河南农业科学,2015,4(4):46-48.
[20] 张瑞奇,胡琳,许为钢,等.黄淮冬麦区不同时期大面积推广品种的高分子量麦谷蛋白亚基组成分析[J].麦类作物学报,2006,26(2):63-67.
[21] 裴自友,温辉芹,张立生,等.富硒黑粒小麦品种冬黑1号的研究[J].种子,2013,32(7):74-76.
[22] 郭明慧,裴自友,温辉芹,等.黑粒小麦冬黑10号的细胞学和麦谷蛋白组成分析[J].农学学报,2012,2(6):27-30,48.
[23] 陈蕴文,张莉莉,余欣欣,等.黄淮麦区南片小麦Glu-3位点等位变异分析[J].华北农学报,2015,4(4):25-30.
[24] 高华利,王黎明,董普辉,等.河南省主推小麦品种籽粒储藏蛋白质遗传多样性分析[J].种子,2015,34(2):80-85.
[25] 范贵强,王化俊,马小乐,等.138份河西地区春小麦种质资源的遗传多样性分析[J].麦类作物学报,2013,33(2):268-272.

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