Sequence Analysis of Brassica napus MAP Kinase 3 and It's Expression Responses to Environmental Signals

doi:10.7668/hbnxb.2016.05.014

Abstract

Abstract: In order to explore the effect of environment signals on the expression of MPK3 in oilseed rape,a full length cDNA of Brassica napus MPK3 (BnMPK3) was cloned from the cv.Zhongshuang No.9 by homology cloning approach.Sequence analysis showed that BnMPK3 contained a highly conserved motif TEY and a CD domain (common docking domain) and was consisted of 370 amino acid residues exhibiting 94% identity with AtMPK3.Transient expression assays in tobacco showed that BnMPK3 is localized in nuclei.Quantitative RT-PCR analysis showed that BnMPK3 was especially expressed in stems,flowers and leaves and revealed that its expression was significantly up-regulated by the treatments with NaCl solution,4℃,PEG4000 solution,K₂Cr₂O₇ solution or MeJA,ACC solution.The results demonstrated that BnMPK3 was a response gene involved in responses to various environmental stresses including high-salinity,low temperature,drought and heavy metal pollution and to signaling molecules JA and ET,suggesting that BnMPK3 may involve in JA and ET signaling pathways in defense response against various environmental stresses in oilseed rape,which will provide useful information in further in-depth functional analysis of BnMPK3 in defense to environmental stresses.

Key words: Brassica napus, BnMPK3, Expression responses, Environmental stresses

CLC Number:

S565.4
Q78

CHEN Ting, FANG Hedi, LI Xiao, LI Guanying, TAN Xiaoli, ZHANG Zhiyan, WANG Zheng. Sequence Analysis of Brassica napus MAP Kinase 3 and It's Expression Responses to Environmental Signals[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 94-100. doi: 10.7668/hbnxb.2016.05.014.

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http://www.hbnxb.net/EN/Y2016/V31/I5/94

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