Abstract: In order to increase improvement efficiency of radish cytoplasmic male sterile (Ogu CMS) restorers in Brassica napus L.,conventional techniques and improved techniques combined conventional techniques with microspore culture and marker-assisted selection (MAS) were studied.Results implied that there were great selection efficiency differences among different screening procedures in segregated populations.One of effective conventional improvement techniques was to cross hybrids with Ogu CMS lines,their progeny without Ogu CMS restorer genes would be sterile,which made selection be more visible at flowering stage and test crosses were not necessary.If modern biological techniques were utilized,the most effective screening procedures were cross,MAS and microspore culture.This procedure could decrease selection time from 8 generations to 3 generations,and experimental labor decreased by 90%.In this study,19 radish specific SCAR markers were screened,which could amplify unique and distinguishable bands in Ogu CMS restorers.However,only 10 radish specific SCAR markers could be screened in all Ogu CMS restorers.Results indicated that the radish specific markers of Ogu CMS restorer R581 were not the same as previous Ogu CMS restorers,the mean similarity ratio of R581 compared with other Ogu CMS restorers were from 48.28% to 93.10%.This study also found that radish specific markers could not distinguish restorers with high glucosinolates content from those with low glucosinolates content,which still need to be screened by analytical devices.In this study,6 Ogu CMS restorers were bred,which had low erucic acid content,low glucosinolates content,cold injury resistance,normal pod bearing rate and fine agronomic traits.These restorers made it feasible for Ogu CMS to be utilized in Brassica napus heterosis in China.

Key words: Brassica napus L., Radish cytoplamic male sterility, Restorer, Improvement, Selection efficiency

CLC Number: 

• S565.403