Abortion Characteristics of NWB Cytoplasm Male Sterile and Genetic Law of Fertility Restoring Genes in Radish

doi:10.7668/hbnxb.20191800

Abstract

Abstract: To explore the abortion characteristics and the genetic law of fertility restoring genes of NWB cytoplasm male sterile (CMS) in radish, 3 NWB CMS lines and 5 restoration materials were used as main test materials. The abortion characteristics of NWB CMS under different nuclear backgrounds were analyzed by paraffin section and scanning electron microscope, and the genetic law of restoring genes was revealed by analysis of fertility segregation of F₁, F₂, BC₁ population between sterile lines and restorer lines. The results showed that the different male sterile lines had different stamen morphology, but the pollen abortion of the CMS lines all started from the stage of mononuclear microspore, and there were differences in the degree of pollen degradation. A2 had shortening filament, shrinking anther with no visible pollen, the structure of A2's anther chamber was wrinkled and deformed, the shells of pollen grains were tightly packed together in a linear shape;A3 had normal filament, plump anther with no visible pollen, A3's anther chamber structure was basically normal, most of the pollen grains were degraded, and only a small amount were attached to the wall of the chamber;A4 had normal filament, plump anther with some nonfunctional pollen, there were a large number of unstained pollen grains in the anther chamber of A4, and the unstained pollen grains were sunken along three germ furrow to form polyhedron. The genetic laws of the 5 NWB CMS restorer materials were further analyzed, and the restorer genes were characterized by complete dominant inheritance. The number of the restorer genes varied with different materials, R1 contained 3 pairs of restorer genes, R2-R4 contained 2 pairs of restorer genes, and R5 contained 1 pair of restorer genes. In summary, NWB CMS stamen abortion morphology was different under different nuclear backgrounds, but the abortion period was the same, and the genetic rules of restoration genes vary from material to material. These results provide a certain theoretical basis for the breeding and utilization of radish NWB CMS.

Key words: Radish, NWB cytoplasm male sterile, Abortion characteristics, Restorer gene, Genetic law

CLC Number:

S631.03

LI Xiaomei, YANG Feng, YONG Xiaoping, CHEN Lin, RAN Ke, RAN Maolin. Abortion Characteristics of NWB Cytoplasm Male Sterile and Genetic Law of Fertility Restoring Genes in Radish[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 90-95. doi: 10.7668/hbnxb.20191800.

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