Genome-wide Identification and Expression Analysis of Genes Related to Gibberellin Biosynthesis and Signal Transduction in Radish

doi:10.7668/hbnxb.20193146

Abstract

Abstract:

Gibberellin pathway is an important pathway in plant flowering regulation.In order to understand the role of gibberellin pathway related genes in the regulation of radish flowering. The structure,physicochemical properties,chromosome distribution,promoter cis-elements and tissue-specific expression of gibberellin biosynthesis and signal transduction related genes in radish were analyzed by bioinformatics.The expression levels of these genes in radish varieties with different florescence were detected by Real-time fluorescence quantitative PCR(qPCR).The results showed there were 46 genes related to gibberellin biosynthesis and signal transduction in radish genome,among them,the gene numbers of CPS,KS,KO,KAO,GA20OX,GA3OX,GA2OX,GAI,RGA,RGL,GID1 and SKP2 were 2,1,2,2,9,5,12,1,1,4,3 and 4,respectively.They were unevenly distributed on 9 chromosomes,molecular weight of their coding proteins were 21.32—127.80 ku,and the isoelectric points of the proteins were from 4.72 to 9.04.The analysis of gene structure and conserved domain showed that the number of exons of these 46 genes ranged from 1 to 21,and some conserved motifs were shared by most genes.Promoter cis-elements analysis showed that the promoters of these 46 genes contained cis-elements related to light,gibberellin,auxin,ABA,SA,low temperature,drought,etc.Using radish gene expression database analysis,it was found that the expression levels of these 46 genes were different in different tissues and at different developmental stages;qPCR detection showed that there were significant differences in the expression of these genes between early flowering material Xinlimei and late flowering material wild radish,suggesting that they may be closely related to the reproductive growth of radish.

Key words: Radish, Gibberellin biosynthesis, Signal transduction, Gene, Expression analysis

YANG Wenlong, LI Xixiang, ZHANG Xiaohui, SONG Jiangping, JIA Huixia, WANG Haiping. Genome-wide Identification and Expression Analysis of Genes Related to Gibberellin Biosynthesis and Signal Transduction in Radish[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 1-14. doi: 10.7668/hbnxb.20193146.

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Figures/Tables 10

References 51

[1]	包崇来, 汪精磊, 胡天华, 胡海娇, 王五宏, 魏庆镇. 我国萝卜产业发展现状与育种方向探讨[J]. 浙江农业科学, 2019, 60(5): 707-710.doi:10.16178/j.issn.0528-9017.20190503. doi: 10.16178/j.issn.0528-9017.20190503
	Bao C L, Wang J L, Hu T H, Hu H J, Wang W H, Wei Q Z. Current situation and breeding direction of radish industry development[J]. Journal of Zhejiang Agricultural Sciences, 2019, 60(5): 707-710.
[2]	Gao S P, Chu C C. Gibberellin metabolism and signaling: Targets for improving agronomic performance of crops[J]. Plant and Cell Physiology, 2020, 61(11): 1902-1911.doi:10.1093/pcp/pcaa104. doi: 10.1093/pcp/pcaa104 pmid: 32761079
[3]	Olszewski N, Sun T P, Gubler F. Gibberellin signaling: Biosynthesis,catabolism,and response pathways[J]. The Plant Cell, 2002, 14(S1): S61-S80.doi:10.1105/tpc.010476. doi: 10.1105/tpc.010476 URL
[4]	Sakamoto T, Miura K, Itoh H, Tatsumi T, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Agrawal G K, Takeda S, Abe K, Miyao A, Hirochika H, Kitano H, Ashikari M, Matsuoka M. An overview of gibberellin metabolism enzyme genes and their related mutants in rice[J]. Plant Physiology, 2004, 134(4): 1642-1653.doi:10.1104/pp.103.033696. doi: 10.1104/pp.103.033696 pmid: 15075394
[5]	Hedden P. The current status of research on gibberellin biosynthesis[J]. Plant and Cell Physiology, 2020, 61(11): 1832-1849.doi:10.1093/pcp/pcaa092. doi: 10.1093/pcp/pcaa092 pmid: 32652020
[6]	Zhao H J, Dong J L, Wang T. Function and expression analysis of gibberellin oxidases in apple[J]. Plant Molecular Biology Reporter, 2010, 28(2): 231-238.doi:10.1007/s11105-009-0146-8. doi: 10.1007/s11105-009-0146-8 URL
[7]	Fleet C M, Sun T P. A DELLAcate balance: The role of gibberellin in plant morphogenesis[J]. Current Opinion in Plant Biology, 2005, 8(1): 77-85.doi:10.1016/j.pbi.2004.11.015. doi: 10.1016/j.pbi.2004.11.015 pmid: 15653404
[8]	Mitchum M G, Yamaguchi S, Hanada A, Kuwahara A, Yoshioka Y, Kato T, Tabata S, Kamiya Y, Sun T P. Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development[J]. The Plant Journal, 2006, 45(5): 804-818.doi:10.1111/j.1365-313X.2005.02642.x. doi: 10.1111/j.1365-313X.2005.02642.x URL
[9]	Wuddineh W A, Mazarei M, Zhang J Y, Poovaiah C R, Mann D G J, Ziebell A, Sykes R W, Davis M F, Udvardi M K, Stewart C N. Identification and overexpression of gibberellin 2-oxidase(GA2ox)in switchgrass(Panicum virgatum L.)for improved plant architecture and reduced biomass recalcitrance[J]. Plant Biotechnology Journal, 2015, 13(5): 636-647.doi:10.1111/pbi.12287. doi: 10.1111/pbi.12287 pmid: 25400275
[10]	Schomburg F M, Bizzell C M, Lee D J, Zeevaart J A D, Amasino R M. Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants[J]. The Plant Cell, 2003, 15(1): 151-163.doi:10.1105/tpc.005975. doi: 10.1105/tpc.005975 URL
[11]	Giacomelli L, Rota-Stabelli O, Masuero D, Acheampong A K, Moretto M, Caputi L, Vrhovsek U, Moser C. Gibberellin metabolism in Vitis vinifera L.during bloom and fruit-set: Functional characterization and evolution of grapevine gibberellin oxidases[J]. Journal of Experimental Botany, 2013, 64(14): 4403-4419.doi:10.1093/jxb/ert251. doi: 10.1093/jxb/ert251 pmid: 24006417
[12]	Yamaguchi S. Gibberellin biosynthesis in Arabidopsis[J]. Phytochemistry Reviews, 2006, 5(1): 39-47.doi:10.1007/s11101-005-4248-0. doi: 10.1007/s11101-005-4248-0 URL
[13]	Han F M, Zhu B G. Evolutionary analysis of three gibberellin oxidase genes in rice,Arabidopsis,and soybean[J]. Gene, 2011, 473(1): 23-35.doi:10.1016/j.gene.2010.10.010. doi: 10.1016/j.gene.2010.10.010 URL
[14]	Kaneko M, Itoh H, Inukai Y, Sakamoto T, Ueguchi-Tanaka M, Ashikari M, Matsuoka M. Where do gibberellin biosynthesis and gibberellin signaling occur in rice plants?[J]. The Plant Journal, 2003, 35(1): 104-115.doi: 10.1046/j.1365-313x.2003.01780.x. doi: 10.1046/j.1365-313x.2003.01780.x URL
[15]	Yamaguchi S. Gibberellin metabolism and its regulation[J]. Annual Review of Plant Biology, 2008, 59: 225-251.doi:10.1146/annurev.arplant.59.032607.092804. doi: 10.1146/annurev.arplant.59.032607.092804 pmid: 18173378
[16]	Davière J M, Achard P. Gibberellin signaling in plants[J]. Development, 2013, 140(6): 1147-1151.doi:10.1242/dev.087650. doi: 10.1242/dev.087650 URL
[17]	Sun T P. Gibberellin-GID1-DELLA: A pivotal regulatory module for plant growth and development[J]. Plant Physiology, 2010, 154(2): 567-570.doi:10.1104/pp.110.161554. doi: 10.1104/pp.110.161554 URL
[18]	Lawit S J, Wych H M, Xu D P, Kundu S M, Tomes D T. Maize DELLA Proteins dwarf plant8 and dwarf plant9 as modulators of plant development[J]. Plant and Cell Physiology, 2010, 51(11): 1854-1868.doi:10.1093/pcp/pcq153. doi: 10.1093/pcp/pcq153 pmid: 20937610
[19]	Ogawa M, Kusano T, Katsumi M, Sano H. Rice gibberellin-insensitive gene homolog,OsGAI,encodes a nuclear-localized protein capable of gene activation at transcriptional level[J]. Gene, 2000, 245(1): 21-29.doi:10.1016/s0378-1119(00)00018-4. doi: 10.1016/s0378-1119(00)00018-4 pmid: 10713441
[20]	Chandler P M, Marion-Poll A, Ellis M, Gubler F. Mutants at the Slender1 locus of barley cv Himalaya.molecular and physiological characterization[J]. Plant Physiology, 2002, 129(1): 181-190.doi:10.1104/pp.010917. doi: 10.1104/pp.010917 pmid: 12011349
[21]	Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J. Slender rice,a constitutive gibberellin response mutant,is caused by a null mutation of the SLR1 gene,an ortholog of the height-regulating gene GAI/RGA/RHT/D8[J]. The Plant Cell, 2001, 13(5): 999-1010.doi:10.1105/tpc.13.5.999. doi: 10.1105/tpc.13.5.999 URL
[22]	Peng J R, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. Green revolution' genes encode mutant gibberellin response modulators[J]. Nature, 1999, 400(6741): 256-261.doi:10.1038/22307. doi: 10.1038/22307 URL
[23]	Silverstone A L, Ciampaglio C N, Sun T P. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway[J]. The Plant Cell, 1998, 10(2): 155-169.doi:10.1105/tpc.10.2.155. doi: 10.1105/tpc.10.2.155 URL
[24]	Lee S, Cheng H, King K E, Wang W, He Y W, Hussain A, Lo J, Harberd N P, Peng J R. Gibberellin regulates Arabidopsis seed germination via RGL2,a GAI/RGA-like gene whose expression is up-regulated following imbibition[J]. Genes & Development, 2002, 16(5): 646-658.doi:10.1101/gad.969002. doi: 10.1101/gad.969002 URL
[25]	Peng J, Carol P, Richards D E, King K E, Cowling R J, Murphy G P, Harberd N P. The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses[J]. Genes & Development, 1997, 11(23): 3194-3205.doi:10.1101/gad.11.23.3194. doi: 10.1101/gad.11.23.3194 URL
[26]	Silverstone A L, Jung H S, Dill A, Kawaide H, Kamiya Y, Sun T P. Repressing a repressor: Gibberellin-induced rapid reduction of the RGA protein in Arabidopsis[J]. The Plant Cell, 2001, 13(7): 1555-1566.doi:10.1105/tpc.010047. doi: 10.1105/tpc.010047
[27]	Tyler L, Thomas S G, Hu J H, Dill A, Alonso J M, Ecker J R, Sun T P. DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis[J]. Plant Physiology, 2004, 135(2): 1008-1019.doi:10.1104/pp.104.039578. doi: 10.1104/pp.104.039578 URL
[28]	Wen C K, Chang C R. Arabidopsis RGL1 encodes a negative regulator of gibberellin responses[J]. The Plant Cell, 2002, 14(1): 87-100.doi:10.1105/tpc.010325. doi: 10.1105/tpc.010325 URL
[29]	Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Y I C, Kitano H, Yamaguchi I, Matsuoka M. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin[J]. Nature, 2005, 437(7059): 693-698.doi:10.1038/nature04028. doi: 10.1038/nature04028 URL
[30]	Murase K, Hirano Y, Sun T P, Hakoshima T. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1[J]. Nature, 2008, 456: 459-463.doi: 10.1038/nature07519. doi: 10.1038/nature07519 URL
[31]	Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M. Structural basis for gibberellin recognition by its receptor GID1[J]. Nature, 2008, 456(7221): 520-523.doi:10.1038/nature07546. doi: 10.1038/nature07546 URL
[32]	Griffiths J, Murase K, Rieu I, Zentella R, Zhang Z L, Powers S J, Gong F, Phillips A L, Hedden P, Sun T P, Thomas S G. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis[J]. The Plant Cell, 2006, 18(12): 3399-3414.doi:10.1105/tpc.106.047415. doi: 10.1105/tpc.106.047415 URL
[33]	Ueguchi-Tanaka M, Nakajima M, Katoh E, Ohmiya H, Asano K, Saji S, Xiang H Y, Ashikari M, Kitano H, Yamaguchi I, Matsuoka M. Molecular interactions of a soluble gibberellin receptor,GID1,with a rice DELLA protein,SLR1,and gibberellin[J]. The Plant Cell, 2007, 19(7): 2140-2155.doi:10.1105/tpc.106.043729. doi: 10.1105/tpc.106.043729 URL
[34]	Lechner E, Achard P, Vansiri A, Potuschak T, Genschik P. F-box proteins everywhere[J]. Current Opinion in Plant Biology, 2006, 9(6): 631-638.doi:10.1016/j.pbi.2006.09.003. doi: 10.1016/j.pbi.2006.09.003 pmid: 17005440
[35]	Hirano K, Asano K, Tsuji H, Kawamura M, Mori H, Kitano H, Ueguchi-Tanaka M, Matsuoka M. Characterization of the molecular mechanism underlying gibberellin perception complex formation in rice[J]. The Plant Cell, 2010, 22(8): 2680-2696.doi:10.1105/tpc.110.075549. doi: 10.1105/tpc.110.075549 pmid: 20716699
[36]	McGinnis K M, Thomas S G, Soule J D, Strader L C, Zale J M, Sun T P, Steber C M. The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase[J]. The Plant Cell, 2003, 15(5): 1120-1130.doi:10.1105/tpc.010827. doi: 10.1105/tpc.010827 URL
[37]	Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Jeong D H, An G, Kitano H, Ashikari M, Matsuoka M. Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant[J]. Science, 2003, 299(5614): 1896-1898.doi:10.1126/science.1081077. doi: 10.1126/science.1081077 pmid: 12649483
[38]	Mitsui Y, Shimomura M, Komatsu K, Namiki N, Shibata-Hatta M, Imai M, Katayose Y, Mukai Y, Kanamori H, Kurita K, Kagami T, Wakatsuki A, Ohyanagi H, Ikawa H, Minaka N, Nakagawa K, Yu S W, Sasaki T. The radish genome and comprehensive gene expression profile of tuberous root formation and development[J]. Scientific Reports, 2015, 5: 10835.doi:10.1038/srep10835. doi: 10.1038/srep10835 pmid: 26056784
[39]	Zhang X H, Liu T J, Wang J L, Wang P, Qiu Y, Zhao W, Pang S, Li X M, Wang H P, Song J P, Zhang W L, Yang W L, Sun Y Y, Li X X. Pan-genome of Raphanus highlights genetic variation and introgression among domesticated,wild,and weedy radishes[J]. Molecular Plant, 2021, 14(12): 2032-2055.doi:10.1016/j.molp.2021.08.005. doi: 10.1016/j.molp.2021.08.005 URL
[40]	吴建明, 陈荣发, 黄杏, 丘立杭, 李杨瑞. 高等植物赤霉素生物合成关键组分GA20-oxidase氧化酶基因的研究进展[J]. 生物技术通报, 2016, 32(7): 1-12.doi:10.13560/j.cnki.biotech.bull.1985.2016.07.001. doi: 10.13560/j.cnki.biotech.bull.1985.2016.07.001
	Wu J M, Chen R F, Huang X, Qiu L H, Li Y R. Studies on the gene of key component GA20-oxidase for gibberellin biosynthesis in plant[J]. Biotechnology Bulletin, 2016, 32(7): 1-12. doi: 10.13560/j.cnki.biotech.bull.1985.2016.07.001
[41]	董凤, 樊胜, 马小龙, 孟媛, 左希亚, 刘小杰, 李珂, 刘桢, 韩明玉, 张东. 苹果赤霉素氧化酶基因GA2ox、GA3ox和GA20ox家族全基因组鉴定及表达分析[J]. 园艺学报, 2018, 45(4): 613-626.doi:10.16420/j.issn.0513-353x.2017-0452. doi: 10.16420/j.issn.0513-353x.2017-0452
	Dong F, Fan S, Ma X L, Meng Y, Zuo X Y, Liu X J, Li K, Liu Z, Han M Y, Zhang D. Genome-wide identification and expression analysis of GA2ox,GA3ox and GA20ox in apple[J]. Acta Horticulturae Sinica, 2018, 45(4): 613-626.
[42]	何红红. 葡萄赤霉素氧化酶基因GA2ox、GA3ox和GA20ox家族的鉴定与GA2ox7的耐盐性功能分析[D]. 兰州: 甘肃农业大学, 2021.doi: 10.27025/d.cnki.ggsnu.2021.000091. doi: 10.27025/d.cnki.ggsnu.2021.000091
	He H H. Identification of GA2ox,GA3ox and GA20ox families of gibberellin oxidase genes and salt tolerance functional analysis of GA2ox7 in Vitis vinifera[D]. Lanzhou: Gansu Agricultural University, 2021.
[43]	Fukazawa J, Mori M, Watanabe S, Miyamoto C, Ito T, Takahashi Y. DELLA-GAF1 complex is a main component in gibberellin feedback regulation of GA20 oxidase 2[J]. Plant Physiology, 2017, 175(3): 1395-1406.doi:10.1104/pp.17.00282. doi: 10.1104/pp.17.00282 pmid: 28916594
[44]	Appleford N E J, Evans D J, Lenton J R, Gaskin P, Croker S J, Devos K M, Phillips A L, Hedden P. Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat[J]. Planta, 2006, 223(3): 568-582.doi:10.1007/s00425-005-0104-0. doi: 10.1007/s00425-005-0104-0 pmid: 16160850
[45]	李长江. 库尔勒香梨花、幼果不同部位内源激素含量的差异分析[D]. 乌鲁木齐: 新疆农业大学, 2016.doi:10.7666/d.Y3101296. doi: 10.7666/d.Y3101296
	Li C J. Analysis on the differences of endogenous hormone contents in different parts of flower and young fruit of Korla fragrant pear[D]. Urumqi: Xinjiang Agricultural University, 2016.
[46]	任秀霞, 王顺利, 薛璟祺, 杨若雯, 管艳忍, 李丹丹, 薛玉前, 张秀新. GA和ABA信号转导关键基因在不同温度处理凤丹牡丹种子中的表达分析[J]. 园艺学报, 2017, 44(1): 80-88.doi:10.16420/j.issn.0513-353x.2016-0374. doi: 10.16420/j.issn.0513-353x.2016-0374
	Ren X X, Wang S L, Xue J Q, Yang R W, Guan Y R, Li D D, Xue Y Q, Zhang X X. Expression analysis of GA and ABA signal transduction genes in Peaonia ostti Fengdan seeds under different temperature treatments[J]. Acta Horticulturae Sinica, 2017, 44(1): 80-88.
[47]	Abelenda J A, Navarro C, Prat S. Flowering and tuberization: A tale of two nightshades[J]. Trends in Plant Science, 2014, 19(2): 115-122.doi:10.1016/j.tplants.2013.09.010. doi: 10.1016/j.tplants.2013.09.010 pmid: 24139978
[48]	Wickland D P, Hanzawa Y. The flowering locus t/terminal flower 1 gene family: Functional evolution and molecular mechanisms[J]. Molecular Plant, 2015, 8(7): 983-997.doi:10.1016/j.molp.2015.01.007. doi: 10.1016/j.molp.2015.01.007 pmid: 25598141
[49]	Qi H D, Lin Y, Ren Q P, Wang Y Y, Xiong F, Wang X L. RNA splicing of FLC modulates the transition to flowering[J]. Frontiers in Plant Science, 2019, 10: 1625.doi:10.3389/fpls.2019.01625. doi: 10.3389/fpls.2019.01625 URL
[50]	Jin S, Nasim Z, Susila H, Ahn J H. Evolution and functional diversification of FLOWERING LOCUS T/TERMINAL FLOWER 1 family genes in plants[J]. Seminars in Cell & Developmental Biology, 2021, 109: 20-30.doi:10.1016/j.semcdb.2020.05.007. doi: 10.1016/j.semcdb.2020.05.007
[51]	Zhu P, Lister C, Dean C. Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression[J]. Nature, 2021, 599(7886): 657-661.doi:10.1038/s41586-021-04062-5. doi: 10.1038/s41586-021-04062-5 URL

基因 Gene	正向引物序列(5'—3') Forward primer sequence (5'—3')			反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAPDH	ACCACTAACTGCCTTGCTCC			AGCTCTTCCACCTCTCCAGT
RsCPS	CCTGCGTCGTTGCTCTAACAT			CTCAAGTAATGAAGGAAATGCCACT
RsKS	CAAGATTGGGATTTGGTTGGT			AGGGATAAACTGTGGGAACTGC
RsKO	TTCCCATTCGCTATGCTCAT			TCATCCAAGAACCTCTCAGGC
RsKAO	CGGTTTGTCATTGGTCACAGT			GAAATCAGCATCCCACATTGAA
RsGA20OX1	CGCTCCATTAGTCCCAACC			GTGAAGTCAGGGTATCTCCTTGCT
RsGA20OX2	GCATAGAGCGGTTGTGAACAG			GCCAAGTGAAATCAGGGTATCTC
RsGA20OX3	TTCCTCCTAACCCTCAATCAGTT			CTCTCTTTCACCAGACATCATTCTC
RsGA20OX4	TCTCTAACCATACTTCAACAAGACTACAGT			TCTCACTATTCACTACCGCCTGAT
RsGA3OX1	GCGTCAGCGAAGAAGACATT			CTTGGAGACCAGCAGTGTTGTT
RsGA3OX2	CGTCGGTGACTTACTCCACATT			TGCTTCCAAGAAAGAGATGGG
RsGA3OX3	CCTCACACTGACTCTTCCCTACTG			GAGACAAATCGCCCATTATGAC
RsGA3OX4	TACGGTAGAGCCGATTCCTG			GGACTTATCTCCACATCACCAGC
基因 Gene		正向引物序列(5'—3') Forward primer sequence (5'—3')	反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAI		GTGTTGGGCTACAAAGTCAGG	GGGTTGAACTCCGTGAGC
RsRGA		GCTCATCTCGCCACGGAT	ACGCTTGTTGTATTGATTCGGA
RsGID1-1		GTTTCCGAAGAGTCTCGTGG	ACGCAGCAATCTCATCCAT
RsGID1-2		CTTGTCGTTGTTGCTGGTTTAGAT	GTGCTTCGGCTACACTCCTCT
RsGID1-3		GACCGTGATTGGTATTGGAGA	CCGCACTCTTGAGCCCTT
RsFLC		ATCCGACATCAATCTTCCG	TACACACACAATCTCTCAGCCA
RsFRI		ATACCTTTGGGATTGAGGATAGG	TCTCTGTGTCTAAGTTGTGAGCCT
RsFT		TGCGGAGGACGAAGAAGTT	TATTATCACCGTTCATTGCTCG

基因 Gene	正向引物序列(5'—3') Forward primer sequence (5'—3')			反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAPDH	ACCACTAACTGCCTTGCTCC			AGCTCTTCCACCTCTCCAGT
RsCPS	CCTGCGTCGTTGCTCTAACAT			CTCAAGTAATGAAGGAAATGCCACT
RsKS	CAAGATTGGGATTTGGTTGGT			AGGGATAAACTGTGGGAACTGC
RsKO	TTCCCATTCGCTATGCTCAT			TCATCCAAGAACCTCTCAGGC
RsKAO	CGGTTTGTCATTGGTCACAGT			GAAATCAGCATCCCACATTGAA
RsGA20OX1	CGCTCCATTAGTCCCAACC			GTGAAGTCAGGGTATCTCCTTGCT
RsGA20OX2	GCATAGAGCGGTTGTGAACAG			GCCAAGTGAAATCAGGGTATCTC
RsGA20OX3	TTCCTCCTAACCCTCAATCAGTT			CTCTCTTTCACCAGACATCATTCTC
RsGA20OX4	TCTCTAACCATACTTCAACAAGACTACAGT			TCTCACTATTCACTACCGCCTGAT
RsGA3OX1	GCGTCAGCGAAGAAGACATT			CTTGGAGACCAGCAGTGTTGTT
RsGA3OX2	CGTCGGTGACTTACTCCACATT			TGCTTCCAAGAAAGAGATGGG
RsGA3OX3	CCTCACACTGACTCTTCCCTACTG			GAGACAAATCGCCCATTATGAC
RsGA3OX4	TACGGTAGAGCCGATTCCTG			GGACTTATCTCCACATCACCAGC
基因 Gene		正向引物序列(5'—3') Forward primer sequence (5'—3')	反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAI		GTGTTGGGCTACAAAGTCAGG	GGGTTGAACTCCGTGAGC
RsRGA		GCTCATCTCGCCACGGAT	ACGCTTGTTGTATTGATTCGGA
RsGID1-1		GTTTCCGAAGAGTCTCGTGG	ACGCAGCAATCTCATCCAT
RsGID1-2		CTTGTCGTTGTTGCTGGTTTAGAT	GTGCTTCGGCTACACTCCTCT
RsGID1-3		GACCGTGATTGGTATTGGAGA	CCGCACTCTTGAGCCCTT
RsFLC		ATCCGACATCAATCTTCCG	TACACACACAATCTCTCAGCCA
RsFRI		ATACCTTTGGGATTGAGGATAGG	TCTCTGTGTCTAAGTTGTGAGCCT
RsFT		TGCGGAGGACGAAGAAGTT	TATTATCACCGTTCATTGCTCG

基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW		等电点 pI	不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsCPS1	Rs468820	R9	22262393~22269809	+	2 451	816	94.25		5.66	44.38	88.68	-0.296
RsCPS2	Rs527850	RUS00633	3085~10083	+	2 430	809	93.64		5.96	44.29	87.52	-0.321
RsKS	Rs448620	R9	467671~474678	-	3 393	1 130	127.80		5.53	45.94	86.68	-0.257
RsKO1	Rs169040	R4	11324717~11326905	+	1 521	506	57.88		6.46	47.65	91.92	-0.274
RsKO2	Rs574920	RUS01337	9122~11305	+	1 539	512	58.50		6.96	47.31	91.99	-0.268
RsKAO1	Rs003450	R1	1829046~1831774	-	1 707	568	65.92		6.46	40.72	84.28	-0.309
RsKAO2	Rs355870	R6	46815059~46817122	+	1 470	489	56.54		9.04	39.29	88.49	-0.273
RsGA20OX1	Rs049910	R2	37179260~37180704	+	1 143	380	43.68		5.90	48.14	73.13	-0.425
RsGA20OX2	Rs092510	R2	10472001~10473401	-	1 140	379	43.24		5.56	43.67	70.74	-0.449
RsGA20OX3	Rs074900	R2	28031441~28032910	-	1 140	379	43.64		5.69	48.49	68.89	-0.495
RsGA20OX4	Rs145650	R3	27582762~27584109	+	1 128	375	42.72		6.57	44.89	72.24	-0.432
RsGA20OX5	Rs601630	R3	27601766~27603107	+	1 122	373	42.50		6.57	43.90	72.63	-0.437
RsGA20OX6	Rs157440	R4	4617142~4618592	+	1 146	381	43.62		5.97	48.02	74.46	-0.427
基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW	等电点 pI		不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsGA20OX7	Rs473380	R9	12625480~12626895	+	1 143	380	43.32	6.27		42.14	74.39	-0.351
RsGA20OX8	Rs481550	R9	24415089~24416702	+	1 074	357	40.10	5.78		36.14	74.82	-0.368
RsGA20OX9	Rs481540	R9	24421848~24423450	+	1 074	357	40.06	6.13		38.90	75.10	-0.361
RsGA3OX1	Rs012590	R1	6333424~6334859	-	1 080	359	40.17	5.89		44.75	86.94	-0.227
RsGA3OX2	Rs103440	R2	280484~282019	+	1 164	387	43.49	6.65		43.22	90.44	-0.128
RsGA3OX3	Rs103430	R2	282791~284121	+	1 056	351	38.59	7.65		42.55	92.45	-0.083
RsGA3OX4	Rs338890	R6	33491105~33492531	-	1 074	357	40.42	7.81		44.36	86.55	-0.279
RsGA3OX5	Rs427250	R8	14209249~14210367	+	735	244	27.82	6.30		65.58	83.85	-0.278
RsGA2OX1	Rs023230	R1	13531883~13533834	+	1 020	339	38.08	6.27		53.13	83.04	-0.331
RsGA2OX2	Rs001270	R1	770957~773364	+	990	329	36.41	6.26		48.87	78.84	-0.308
RsGA2OX3	Rs570400	R2	1012769~1014452	+	963	320	35.56	6.66		36.74	81.88	-0.245
RsGA2OX4	Rs052360	R2	34947056~34947691	+	558	185	21.64	8.48		39.32	74.27	-0.404
RsGA2OX5	Rs170920	R4	12212866~12214381	+	984	327	36.72	5.45		31.45	86.64	-0.237
RsGA2OX6	Rs161090	R4	6661715~6662376	-	555	184	21.32	8.47		34.76	72.61	-0.398
RsGA2OX7	Rs268140	R5	33513916~33514668	+	648	215	23.66	5.93		42.74	84.28	-0.120
RsGA2OX8	Rs267680	R5	34128058~34130208	+	999	332	37.95	8.87		47.95	73.01	-0.337
RsGA2OX9	Rs357280	R6	47701447~47704379	+	981	326	36.78	6.28		47.44	80.03	-0.342
RsGA2OX10	Rs419290	R8	7392543~7394486	+	1 023	340	38.23	7.63		47.66	86.50	-0.286
RsGA2OX11	Rs430940	R8	14100094~14100849	+	555	184	21.33	8.16		31.52	73.10	-0.424
RsGA2OX12	Rs449910	R9	1082416~1083847	+	762	253	27.97	5.10		33.98	88.89	-0.155
RsGAI	Rs493610	R9	33197399~33199156	+	1 758	585	64.02	5.48		44.67	79.38	-0.276
RsRGA	Rs179530	R4	17734690~17736416	+	1 599	532	58.15	5.57		45.99	81.79	-0.188
RsRGL1	Rs094860	R2	8444132~8445667	-	1 452	483	53.31	5.38		41.44	91.86	-0.193
RsRGL2	Rs220460	R5	916848~918476	-	1 629	542	59.02	4.72		44.83	83.82	-0.224
RsRGL3	Rs399820	R7	3634445~3636031	+	1 587	528	58.01	4.87		48.52	86.14	-0.207
RsRGL4	Rs541920	RUS00210	27721~29301	+	1 581	526	57.81	4.87		48.77	85.74	-0.226
RsGID1-1	Rs221810	R5	1751276~1752658	+	1 059	352	39.41	6.67		43.00	86.65	-0.259
RsGID1-2	Rs218880	R4	49898901~49900165	+	1 083	360	40.45	6.70		43.51	89.25	-0.139
RsGID1-3	Rs170080	R4	11981312~11982923	-	1 032	343	38.23	6.09		46.41	88.05	-0.176
RsSKP2-1	Rs388660	R7	9192014~9193640	-	1 077	358	39.15	6.86		43.79	99.94	0.059
RsSKP2-2	Rs380870	R7	16044907~16046864	+	1 089	362	39.85	7.76		46.10	97.24	-0.004
RsSKP2-3	Rs414970	R8	4640621~4642318	+	1 095	364	40.05	7.06		42.90	99.12	0.067
RsSKP2-4	Rs415000	R8	4664912~4666661	+	1 122	373	41.13	6.77		47.42	97.42	0.024

基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW		等电点 pI	不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsCPS1	Rs468820	R9	22262393~22269809	+	2 451	816	94.25		5.66	44.38	88.68	-0.296
RsCPS2	Rs527850	RUS00633	3085~10083	+	2 430	809	93.64		5.96	44.29	87.52	-0.321
RsKS	Rs448620	R9	467671~474678	-	3 393	1 130	127.80		5.53	45.94	86.68	-0.257
RsKO1	Rs169040	R4	11324717~11326905	+	1 521	506	57.88		6.46	47.65	91.92	-0.274
RsKO2	Rs574920	RUS01337	9122~11305	+	1 539	512	58.50		6.96	47.31	91.99	-0.268
RsKAO1	Rs003450	R1	1829046~1831774	-	1 707	568	65.92		6.46	40.72	84.28	-0.309
RsKAO2	Rs355870	R6	46815059~46817122	+	1 470	489	56.54		9.04	39.29	88.49	-0.273
RsGA20OX1	Rs049910	R2	37179260~37180704	+	1 143	380	43.68		5.90	48.14	73.13	-0.425
RsGA20OX2	Rs092510	R2	10472001~10473401	-	1 140	379	43.24		5.56	43.67	70.74	-0.449
RsGA20OX3	Rs074900	R2	28031441~28032910	-	1 140	379	43.64		5.69	48.49	68.89	-0.495
RsGA20OX4	Rs145650	R3	27582762~27584109	+	1 128	375	42.72		6.57	44.89	72.24	-0.432
RsGA20OX5	Rs601630	R3	27601766~27603107	+	1 122	373	42.50		6.57	43.90	72.63	-0.437
RsGA20OX6	Rs157440	R4	4617142~4618592	+	1 146	381	43.62		5.97	48.02	74.46	-0.427
基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW	等电点 pI		不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsGA20OX7	Rs473380	R9	12625480~12626895	+	1 143	380	43.32	6.27		42.14	74.39	-0.351
RsGA20OX8	Rs481550	R9	24415089~24416702	+	1 074	357	40.10	5.78		36.14	74.82	-0.368
RsGA20OX9	Rs481540	R9	24421848~24423450	+	1 074	357	40.06	6.13		38.90	75.10	-0.361
RsGA3OX1	Rs012590	R1	6333424~6334859	-	1 080	359	40.17	5.89		44.75	86.94	-0.227
RsGA3OX2	Rs103440	R2	280484~282019	+	1 164	387	43.49	6.65		43.22	90.44	-0.128
RsGA3OX3	Rs103430	R2	282791~284121	+	1 056	351	38.59	7.65		42.55	92.45	-0.083
RsGA3OX4	Rs338890	R6	33491105~33492531	-	1 074	357	40.42	7.81		44.36	86.55	-0.279
RsGA3OX5	Rs427250	R8	14209249~14210367	+	735	244	27.82	6.30		65.58	83.85	-0.278
RsGA2OX1	Rs023230	R1	13531883~13533834	+	1 020	339	38.08	6.27		53.13	83.04	-0.331
RsGA2OX2	Rs001270	R1	770957~773364	+	990	329	36.41	6.26		48.87	78.84	-0.308
RsGA2OX3	Rs570400	R2	1012769~1014452	+	963	320	35.56	6.66		36.74	81.88	-0.245
RsGA2OX4	Rs052360	R2	34947056~34947691	+	558	185	21.64	8.48		39.32	74.27	-0.404
RsGA2OX5	Rs170920	R4	12212866~12214381	+	984	327	36.72	5.45		31.45	86.64	-0.237
RsGA2OX6	Rs161090	R4	6661715~6662376	-	555	184	21.32	8.47		34.76	72.61	-0.398
RsGA2OX7	Rs268140	R5	33513916~33514668	+	648	215	23.66	5.93		42.74	84.28	-0.120
RsGA2OX8	Rs267680	R5	34128058~34130208	+	999	332	37.95	8.87		47.95	73.01	-0.337
RsGA2OX9	Rs357280	R6	47701447~47704379	+	981	326	36.78	6.28		47.44	80.03	-0.342
RsGA2OX10	Rs419290	R8	7392543~7394486	+	1 023	340	38.23	7.63		47.66	86.50	-0.286
RsGA2OX11	Rs430940	R8	14100094~14100849	+	555	184	21.33	8.16		31.52	73.10	-0.424
RsGA2OX12	Rs449910	R9	1082416~1083847	+	762	253	27.97	5.10		33.98	88.89	-0.155
RsGAI	Rs493610	R9	33197399~33199156	+	1 758	585	64.02	5.48		44.67	79.38	-0.276
RsRGA	Rs179530	R4	17734690~17736416	+	1 599	532	58.15	5.57		45.99	81.79	-0.188
RsRGL1	Rs094860	R2	8444132~8445667	-	1 452	483	53.31	5.38		41.44	91.86	-0.193
RsRGL2	Rs220460	R5	916848~918476	-	1 629	542	59.02	4.72		44.83	83.82	-0.224
RsRGL3	Rs399820	R7	3634445~3636031	+	1 587	528	58.01	4.87		48.52	86.14	-0.207
RsRGL4	Rs541920	RUS00210	27721~29301	+	1 581	526	57.81	4.87		48.77	85.74	-0.226
RsGID1-1	Rs221810	R5	1751276~1752658	+	1 059	352	39.41	6.67		43.00	86.65	-0.259
RsGID1-2	Rs218880	R4	49898901~49900165	+	1 083	360	40.45	6.70		43.51	89.25	-0.139
RsGID1-3	Rs170080	R4	11981312~11982923	-	1 032	343	38.23	6.09		46.41	88.05	-0.176
RsSKP2-1	Rs388660	R7	9192014~9193640	-	1 077	358	39.15	6.86		43.79	99.94	0.059
RsSKP2-2	Rs380870	R7	16044907~16046864	+	1 089	362	39.85	7.76		46.10	97.24	-0.004
RsSKP2-3	Rs414970	R8	4640621~4642318	+	1 095	364	40.05	7.06		42.90	99.12	0.067
RsSKP2-4	Rs415000	R8	4664912~4666661	+	1 122	373	41.13	6.77		47.42	97.42	0.024

基序 Motif	E-值 E-value	长度/bp Length	序列 Sequence
GA合成上游基因编码蛋白基序Motifs of GA up-stream synthesis protein
1	9.10E-39	41	PISLTAYREAKEDTEIGGYYIPAGPEVATWIAGVHLDPKRW
2	9.40E-21	21	VLPFGLGEHLCIGBRLASLGI
3	3.70E-27	29	ERJITTLACVVALTEWNIYELAYHKGLQF
4	3.50E-19	29	YVHRYWTDKGICWARCSHVQDIDDTAMAF
5	3.60E-22	29	DEKGKTLEREEIFDVLLVELVEGAVDTDH
6	1.50E-19	29	SYEIHKGLPLIGALPVQGEEAPHISIGYI
7	8.00E-32	50	EIARGPNIDVPKDSPVLKDJYAKKELKLTRDPDDFHKKIPRCJLNGLLGA
8	3.40E-35	50	DWLIEWGLSKLELHERDHPQEPVNFEAEREHERFGWAKKQVFGKDIFSIH
9	4.30E-30	50	QYGGENDVWIGKTLYRMPYVNNNEYLELAKQDYNNCQALHQLEWDTFKKW
10	3.50E-25	50	GEEGEYFPPGGQGWPFIGGMFNFLRAFKLADPDEFIRKNAKEYGSKGLHK
GA合成下游基因编码蛋白基序Motifs of GA down-stream synthesis protein
1	1.80E-373	29	WVSVPPBPSALVVNIGDTFQALSNGRFKSMEME
2	5.60E-359	29	JGLGPHTDPTILTILHQBNVGGLQVFVDBMEME
3	6.00E-409	50	AKRLIVEACEKHGFFLVVNHGVPEELJSRAEHEMVSFFDLPASEKLKAARMEME
4	1.90E-209	21	FEDNDSILRLNHYPPCPEPDLMEME
5	8.90E-218	25	HRAVVNSESERKSMAFFLCPKLDKVMEME
6	4.30E-228	41	GQEYEDFGKVVZEYCEAMNKLSLKIMELLGESLGIERRDFRMEME
7	1.30E-115	15	LYPDFTWSEYLEFTQMEME
8	3.10E-154	29	PGESSGYASSFIGRFSTKLPWKETLSFKFMEME
9	6.40E-81	31	IPQQFIWPDHEKPSSEVPELQVPLIDLAGFLMEME
10	2.10E-67	29	EPFGYGSKRIGPNGDVGWIEYJLLNANPQMEME
GA信号转导基因编码蛋白基序Motifs of GA signaling protein
1	1.80E-221	50	EEGLQMHFYESCPYLKFAHFTANQAILEALHTASGVHVJDMSLCQGLQWPMEME
2	4.90E-135	49	PPSQDRVMSEVYLGRQILNLVAAEGADRVERHETLSQWRNRFGSAGFDPMEME
3	6.00E-146	41	VKSLKPKIVTVVEQEARHBGPVFLDRFVEAJHYYSSLFDSLMEME
4	3.00E-112	41	KQASMLLALFGGGDGYRVEENEGCLMLGWQTRPLIATSAWKMEME
5	2.00E-97	50	DLCGCVLITDESVVALADWCVHLRSLGLYYCRNITDRAMYSLAQSGVKNKMEME
6	4.20E-89	50	LNLCGCVEAVSDNALZAIGENCNQMQSLNLGWCEKISDDGVMSLAYGCPDMEME
7	2.20E-86	50	WKDIPVELLMRILNLVDDRTVIIASGVCSGWRDAISFGLTRLRLSWCKKNMEME
8	1.40E-75	21	SAMRKVADYFAEALARRIYRLMEME
9	9.60E-76	50	SDGLQELGWKLAQLAQAIGVEFEFKGLVAESLTDLEPEMFETRPESETLVMEME
10	7.60E-73	32	NMDELLAVLGYKVRSSDMADVAQKLEQLEVVLMEME

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