Preliminary Molecular Identification of Ogu CMS Restorers 1575R in Brassica nupus

doi:10.7668/hbnxb.2014.06.008

Abstract

Abstract: The objective of this study is to make molecular identification of 13 Ogu CMS restorers(1575R) in Brassica nupus with restorer gene Rfo and its specific markers to provide basis for different restorers from foreign restorers. Rfo specific marker,linkage markers and specific markers of SRF lines are used to test 1575R with the negative control of 4 maintainer lines and 4 sterile lines.Testing results are used to compare 1575R with foreign restorers RRH1,R113,R2000,SRF lines and NW1717.All the 13 restorers of 1575R lines contain the restorer gene Rfo.1575R lines lack the marker BolJon which is all owned by RRH1,R113,R2000.1575R contained the same specific markers with NW1717.1575R may be different from RRH1,R113,R2000,SRF lines and have a close homology with NW1717.

Key words: Ogu CMS, Restorer gene Rfo, Molecular markers

CLC Number:

Q78
S635.03

XU Ting, DONG Zhen-sheng, ZHANG Bo, GAO Yong-xiang, DUAN Hai-feng, HUANG Wei-nan, DONG Jun-gang. Preliminary Molecular Identification of Ogu CMS Restorers 1575R in Brassica nupus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 40-44. doi: 10.7668/hbnxb.2014.06.008.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.06.008

http://www.hbnxb.net/EN/Y2014/V29/I6/40

References

[1] Ogura H.Studies on the new male sterility in Japanese radish,with special references to the utilization of this sterility towards the practical raising of hybrid seed [J].Mem Fac Agric Kagoshima Univ,1968,6(2):39-78.
[2] Brown G G,Formanova N,Jin H,et al.The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats[J].Plant Journal,2003,35(2):262-272.
[3] Desloire S,Gherbi H,Laloui W,et al.Identification of the fertility restoration locus,Rfo,in radish,as a member of the pentatricopeptide-repeat protein family[J].EMBO Reports,2003,4(6):588-594.
[4] Bonhomme S,Budar F,Lancelin D,et al.Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in hybrids[J].Molecular & General Genetics,1992,235(2):340-348.
[5] 王保仁,常桂菊,戴玉池.甘蓝型油菜萝卜细胞质雄性不育系的研究[J].中国油料,1989(4):3-6,8,105.
[6] Bannerot H,Boudidard L,Chupeau Y.Unexpected difficulties Met with the radish cytoplasm in B.oleraea [J].Eucmpia Crucifeme Newsletter,1977(2):16.
[7] 王俊生,范小芳,谭光轩,等.甘蓝型油菜萝卜质雄性不育恢复系的选育[J].分子植物育种,2011,9(5):617-622.
[8] Pelletier G,Primard C,Vedel F et al.Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion [J].Molecular Genetics and Genomics,1983,191:244-250.
[9] Delourme R,Bouchereau A,Hubert N,et al.Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed(Brassica napus L.)[J].Theoretical and Applied Genetics,1994,88(4):741-748.
[10] Giancola S,Marhadour S,Desloire S,et al.Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed,using the Arabidopsis genome sequence and radish genetic mapping[J].Theoretical and Applied Genetics,2003,107(8):1442-1451.
[11] Primard-Brisset C,Poupard J P.A new recombined double low restorer line for the Ogu-INRA cms in rapeseed(Brassica napus L.) [J].Theoretical and Applied Genetics,2005(111):736-746.
[12] 先锋国际良种公司.具有缩短的Raphanus片段(SRF)的新的芸薹属Ogura恢复系[P].中国专利,200980109801.9,2011-06-01.
[13] 李旭峰,杨毅,王幼平,等.油菜染色体工程研究-蓝花子特性导入油菜[J].四川大学学报:自然科学版,1995,5(5):599-604.
[14] 文雁成,张书芬,王建平,等.甘蓝型油菜萝卜质雄性不育恢复系的筛选和初步研究[J].华北农学报,2010,4(4):102-106.
[15] Chen W J,Li M,Wang T H,et al.Development of new restorer materials with Ogu CMS in Brassica napus [J].Agricultural Science & Technology,2013,14(1):18-25.
[16] 王一峰,董振生,董军刚,等.甘蓝型油菜细胞质雄性不育材料1575A的分子鉴定[J].西北植物学报,2010,30(9):1760-1765.
[17] Hu X E,Sullivan-Gilbert M,Kubik T,et al.Mapping of the ogura fertility restorer gene Rfo and development of Rfo allele-specific markers in canola (Brassica napus L.)[J].Molecular Breeding,2008,22(4):663-674.

Please choose a citation manager

Content to export