Abstract: In order to construct CHX-GFP fusion gene and expressed it in the calli of carota,fusion PCR was used because it needed not analysis restriction enzyme site of DNA fragments and took less labor and time.The plant expression vector of p1301 -35S-CHX-GFP-Nos was constructed by recombinant technology.Using 1/2MS+AS 100 μmol/L and MS+6-BA 0.5 mg/L+2,4-D 0.5 mg/L+agar 6 g/L+sucrose 30 g/L+glucose 10 g/L+AS 50 μmol/L as infection and co-cultivation medium respectively the vector was transformed into the cells of calli after 4 duration co-cultivation with agro-bacterium (EHA105).The CHX-GFP was expressed transiently in the calli with frequence on average was up to 75%.These results would provide foundation for further investigation of CHX sub-cell localization and gene function.

Key words: CHX, GFP, Fusion gene

CLC Number: 

• S631.03

• Q78