Cloning and Expression Analysis of TaAP1-3 Genes of Three Pistils Mutation in Wheat

doi:10.7668/hbnxb.2014.06.006

Abstract

Abstract: To investigate the role of TaAP1-3 in the formation of wheat three pistils trait.Three homologous genes TaAP1-3a, TaAP1-3b and TaAP1-3c were cloned from wheat three pistils near-isogenic lines CM28 and CM28TP in this study.By nucleotide sequence,amino acid sequence,quantification and clustering analysis showed that the cloned cDNA of TaAP1-3a, TaAP1-3b and TaAP1-3c were 1 210,1 208,1 199 bp in length,respectively. TaAP1-3a, TaAP1-3b and TaAP1-3c genes respectively contained an 825,816,855 bp ORF which encode 274,271,284 amino acids.The cDNA sequence of TaAP1-3a, TaAP1-3b and TaAP1-3c with TaAP1-3 were respectively 96.02%,93.1% and 93.56% similarity.And the identities to TaAP1-3 in amino acids sequences were 99%,95%,97% respectively.Cluster analysis showed that TaAP1-3a, TaAP1-3b and TaAP1-3c were clustered into FUL2 clade,and showed greater similarity to A class function genes,such as, TaAGL29, OsMADS15, ZAP1, BM8, EnWM8E and TaAP1-3 genes.Real-time analysis showed the expression level of TaAP1-3a, TaAP1-3b and TaAP1-3c in CM28 and CM28TP were significantly different.For CM28,high expression level was shared in two ridge until floret differentiation (spikelet length 2-5 mm).However,the expression level was higher in the pistil and stamen primordium (spikelets length 5-7mm) of CM28TP.Experimental analysis indicated that the genes of TaAP1-3a, TaAP1-3b, TaAP1-3c may have similar function with A class function genes of above-mentioned,and the difference of expression pattern in CM28 and CM28TP may be bound up with three-pistil traits.The results make the foundation to further explore their roles in forming three-pistil traits in wheat.

Key words: Wheat, Floral organ development, TaAP1-3, MADS-box gene, Gene expression

CLC Number:

S512.1.1
Q78

WANG Yu-wei, PENG Zheng-song, YANG Zai-jun, WEI Shu-hong, LIAO Ming-li, ZHAO Huan, YANG Hui, YANG Yu-feng, WANG Qing-hai. Cloning and Expression Analysis of TaAP1-3 Genes of Three Pistils Mutation in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 28-35. doi: 10.7668/hbnxb.2014.06.006.

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