Molecular Marker Localization of Powdery Mildew Resistance Gene in Wheat Germplasm Line Shannong 10103

doi:10.7668/hbnxb.2015.S1.003

Abstract

Abstract: To identify the genetic characteristic of powdery mildew resistance gene in wheat genotype Shannong 10103,F₂ segregation population derived from the cross between Shannong 10103 and Huixianhong was created and molecular marker analysis was carried out in this study.The results showed that Shannong 10103 and Shannong 10103 Huixianhong F₁ were resistant to powdery mildew in seedlings,and the segregation ratio of resistant and susceptible plants in F₂ was 3 : 1.All these results suggested that the powdery mildew resistance in Shannong 10103 was controlled by a dominant gene,named PML10103.Through screening 2 606 pairs of SSR,EST-SSR and STS primers for polymorphism in the parents and preferred group,seven SSR,EST-SSR markers (Xcinau188,XCAU127,CFE164,CWM109,SWES231,Xcnl113 and SWES1015) were linked to PML10103.The genetic distance between the powdery mildew resistance gene and the SSR,EST-SSR markers-Xcinau188,XCAU127,CFE164,CWM109,SWES231,Xcnl113 and SWES1015 were 0.4,0.7,4.8,3.0,4.9,3.3,0.9 cM,respectively.Take the advantage of the known molecular linkage map, PML10103 was mapped on wheat chromosome 6B.

Key words: Wheat, Shannong 10103, Powdery mildew resistance genes, Molecular markers localization

CLC Number:

S435.12

WU Xia, ZHANG Chao, ZHANG Ming, XU Lin-tao, WANG Hong-gang. Molecular Marker Localization of Powdery Mildew Resistance Gene in Wheat Germplasm Line Shannong 10103[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 19-23. doi: 10.7668/hbnxb.2015.S1.003.

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