Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala

doi:10.7668/hbnxb.2015.S1.002

Abstract

Abstract: DNA methylation plays an important role in regulating plant growth,and it is necessary for plant normal growth and development.In this experiment,methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9^# and the change of DNA methylation status in 9^# after high-voltage electric field.The correlation was discussed between seed degradation phenomena of self-incompatible line and epigenetic one.The results were shown as follows:After high-voltage electric field treatment,both methylation and demethylation occurred;and demethylation seemed to be predominant,the proportion of full-methylation increased slightly and semi-methylation declined clearly and brought about the result of total methylation declined;the physiological indexes of 9^# in seed germination,which showed high-voltage electric field,could promote seed germination and seeding growth and improve the quality of seeds.High-voltage electric field was an effective means to delay and restore growth vigor,and to maintain the quality of self-crossed progeny.

Key words: Brassica oleracea var.acephala, Self-incompatibility, DNA methylation, High-voltage electric field

CLC Number:

Q78
S635.03

ZHANG Yang, HU Zhong-ying, LI Na, DING Bing, WANG Jiang, XIE Li-nan. Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 8-18. doi: 10.7668/hbnxb.2015.S1.002.

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