Inheritance and Variations of DNA Methylation in Interspecific Hybrid F1 Between O.sativa and O.officinalis

doi:10.7668/hbnxb.2017.04.004

Abstract

Abstract: Aiming to analysis the characteristics of DNA methylation during the development processes of leaf and spikelet to explore the effect of genome shock in different tissues of hybrid F₁ between O. sativa and O. officinalis,moreover,the possibility of biological effects,in this study,using the methylation sensitive amplified polymorphism technology (MSAP),patterns and levels of global genomic DNA methylation in leaf and spikelet from parents to interspecific hybrid F₁ were investigated. The results showed that,the similar change trends of DNA methylation level in tissues of leaf and spikelet were observed in all the experimental materials,that is,along with the plant growth and development the DNA methylation level was on a downward trend,and the full methylation level was significantly higher than hemi-methylation level in both leaf and spikelet(P=0.000). In hybrid leaf tissue at the tillering stage, meiosis stage of pollen mother cells and flowering stage,the average total methylation level,full methylation level and hemi-methylation level was 20.19%,16.06% and 4.13%,respectively,which was higher than that of the two parents;while in hybrid spikelet tissue at the meiosis stage of pollen mother cells, microspore development stage and matured pollen grain stage,the average level of total,full and hemi-methylation was 17.38%,13.67% and 3.71%,respectively,which was higher than that of Liaojing 944 and lower than O. officinalis. However,only hemi-methylation level of hybrid was significantly higher than Liaojing 944(P=0.015). DNA methylation patterns in leaf and spikelet were very similar in parents and hybrid. Sequence analysis of 94 differentially methylated fragments showed that 38 fragments were sequence similarity in the NCBI database. The results would be helpful for further analysis of hybrid sterility between O. sativa and O. officinalis.

Key words: Oryzasativa, Oryza officinalis, Interspecific hybrid, DNA methylation, Methylation sensitive amplified polymorphism (MSAP)

CLC Number:

S511.03

HE Ping, SHU Mian, CAI Xiaodan, FU Xuelin. Inheritance and Variations of DNA Methylation in Interspecific Hybrid F₁ Between O.sativa and O.officinalis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 19-31. doi: 10.7668/hbnxb.2017.04.004.

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