Molecular Cloning and Function Analysis of a GmCHI1 Promoter

doi:10.7668/hbnxb.2017.04.005

Abstract

Abstract: To investigate the molecular mechanism of the Class Ⅰ Chitinase gene response to virous stresses,the Class I Chitinase promoter was isolated from soybean by PCR and its sequencing analysis indicated that the amplified fragment (1 641 bp) was 99.8% homologous to the ones reported in the GenBank database. The results showed that it included several conserved stress-responsive elements. By using the expression vector pCAMBIA1391Z without promoter upstream of GUS gene, GmCHI1p was fused in the frame with the GUS reporter gene and the resulting construct pCAM-GmCHI1p was introduced into tobacco by Agrobacterium -mediated method. GUS activity can be detected in the GmCHI1p-transfomants but not untransformed calli (control). The determinations of the GUS activities in the transgenic tobacco plants indicated that GmCHI1p could drive the GUS reporter gene to express in the roots specifically and could be significantly induced by wounding. The GmCHI1 promoter was root tissue-specific and wounding-inducible in its expression. The wounding-inducible expression was occurred only in the wounding tissue or at round it but not transmitted for a long distance. It is expected to have great application prospects in the molecular breeding of transgenic insect resistance.

Key words: Chitinase, Promoter, Root-specific, Hurt-inducible

CLC Number:

Q78
S565.03

ZHAO Chunmei, FAN Xi, XUE Rengao. Molecular Cloning and Function Analysis of a GmCHI1 Promoter[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 32-36. doi: 10.7668/hbnxb.2017.04.005.

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