Effects of 5-Azacytidine on Drought Tolerance of Rice with High Expression of C4-PEPC

doi:10.7668/hbnxb.20191916

Abstract

Abstract: In order to reveal the effect of DNA methylation under drought stress toward rice with high expression of C₄-PEPC (PC), germination test, hydroponics experiments and pot experiments respectively were used in this study. By introducing different concentrations of DNA methylation inhibitor (5-Azacytidine, 5-azaC), the drought simulation treatments combining with the inhibitors were used for the stages of seed germination, seedling and late growth, respectively. The changes including germination rate of seed, the relative water content, malondialdehyde content, proline content, soluble sugar, soluble protein content, PEPC activity and the gene expression levels of related genes, C₄-PEPC genes, SnRK1s gene, and methyltransferase 1 genes of rice leaves at seedling stage, the photosynthesis characteristics of flag leaves at flowering stage were measured, and their yield component were investigated after harvest as well. The results showed that:In bud and seedling stages, the effect of 5-azaC on drought stress present dose effect in rice as following:promoting under low concentrations and inhibiting under high concentrations, the concentrations of the bud stageOsMET1b on this concentration of 5-azaC, and the expression of C₄-PEPC in PC also presented the dose effect. In brief, DNA methylation was involved in rice drought response, but the effects were different in different growth stages, and sugar signal might enhance drought tolerance of PC by participating in DNA methylation.

Key words: High expression of maize C₄-PEPC transgenic rice, 5-Azacytidine, Drought stress, DNA methylation

CLC Number:

YAN Ting, LI Xia, CAO Yue, WU Bohan, WANG Jing, ZHANG Manman. Effects of 5-Azacytidine on Drought Tolerance of Rice with High Expression of C₄-PEPC[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 96-107. doi: 10.7668/hbnxb.20191916.

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