MSAP Analysis of Epigenetic in Peanut under Ralstonia solanacearum Induced

doi:10.7668/hbnxb.2015.02.017

Abstract

Abstract: A high sensitive variety Zhonghua 12 and a high resistance variety Yuanza 9102, infected by R.solanacearum, were used to assess the level and changing pattern of DNA methylation by methylation-sensitive amplified polymorphism (MSAP) technique.The results showed that the level of global DNA methylation of the two varieties were all 35.1% under normal condition and after infection 12 hours, the level of Zhonghua 12 decreased to 31.3% while the level of Yuanza 9102 increased to 37.2%.Methylation and demethylation of DNA of the two varieties were 5.9%, 12.4%, 18.3% and 11.9% after infection.It indicated that the genomic DNA methylation status of the highly resistance variety of peanut were changed in some site after infection by R.solanacearum and changed the expression of some genes for initiating stress mechanism.

Key words: Peanut, R.solanacearum, DNA methylation, MASP

CLC Number:

S565.2

XU Zhi-jun, HUANG Li, JIANG Hui-fang. MSAP Analysis of Epigenetic in Peanut under Ralstonia solanacearum Induced[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 93-99. doi: 10.7668/hbnxb.2015.02.017.

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