MSAP Analysis of DNA Methylation Alteration in Synthetic Hexaploid Wheat

doi:10.3969/j.issn.1000-7091.2012.02.015

Abstract

Abstract: DNA methylation is known to play an important role in the regulation of gene expression in higher?plants． In this study，we assessed the extent and patterns of cytosine methylation alterations in a stable synthetic hexaploid wheat and their progenitor parents using MSAP． Results showed that the levels of methylation in SCA/SQ?(AABBDD) and the parents were 28． 21%，27． 53%，24． 11%，respectively． A total of 1 087 fragments were differentially amplified from the two digests for at least one of the parental lines，about 21． 53% of loci shown changes in?methylation patterns in the allopolyploid． Among these loci，18． 95% were hypermethylated，2． 58% were demethylated． The increased methylation level in synthetic hexaploid wheat compared to its parents might mitigate the shocks?from the hybridization and duplication．

Key words: Synthetic hexaploid wheat, Allopolyploidy, DNA methylation, MSAP

CLC Number:

S512.01

NIE Li-hong, WANG Yan-zhao, SUN Qi-xin, FANG Wei-ping, XIE De-yi. MSAP Analysis of DNA Methylation Alteration in Synthetic Hexaploid Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 77-80. doi: 10.3969/j.issn.1000-7091.2012.02.015.

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