MSAP Analysis of Epigenetics of the Same Genotypes of Flue-cuRed Tobacco in DiffeRent Ecological Regions

doi:10.3969/j.issn.1000-7091.2013.01.006

Abstract

Abstract: The same flue-cuRed tobacco vaRiety in diffeRent ecological tobacco aRea has a diffeRent style of flavoR， to Reveal the mechanism of the flavoR style foRmation， flue-cuRed tobacco K326 as the mateRial，we used MSAP to analysis tabacco genomic DNA which was in faint scent and thick aRoma ecological zones． The Results showed that the Yongzhou tobacco leaves methylation level is significantly higheR than the level of methylation of Yuxi，of which the hemimethylated Ratio is 3． 97 peRcentage point higheR， full methylation Ratio is 1． 74 peRcentage point higheR; MSAP Ratio is 5． 78% higheR; BLAST compaRison showed that the MSAP diffeRences fRagment has a high degRee of homology with two flue-cuRed tobaccon functional gene．

Key words: Flue-cuRed tobacco, Ecological Regions, Epigenetics, Methylation, MSAP

CLC Number:

S572

LI Hui, SUN Huan-liang, LI De-fang. MSAP Analysis of Epigenetics of the Same Genotypes of Flue-cuRed Tobacco in DiffeRent Ecological Regions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 32-36. doi: 10.3969/j.issn.1000-7091.2013.01.006.

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References

1] BiRd A． DNA methylation patteRns and epigenetic memoRy
[J]． Genes Dev， 2002， 16( 1) : 6 - 21．

[2] Rassoulzadegan M，GRandjean V，Gounon P，et al． RNAmediated non-mendelian inheRitance of an epigenetic change in the mouse[J]． NatuRe， 2006， 441( 7092) : 469 - 474．

[3] Saze H，Mittelsten S O，Paszkowski J． Maintenance of CpG methylation is essential foR epigenetic inheRitance duRing plant gametogenesis[J]． Nat Genet， 2003， 34: 65 - 69．

[4] GaRcia R N，Dcvila M F，Robe L J，et al． FiRst evidence of methylation in the genome of DRosophil a willistonⅠ[J]． Genetica， 2007， 131( 1) : 91 - 105．

[5] Zhao X X，Chai Y，Liu B． Epigenetic inheRitance and vaRiation of DNA，methylation level and patteRn in maize intRa- specific hybRids[J]． Plant Sci，2007，172 ( 5) : 930 - 938．

[6] PeRedo E L，Revilla M，ARRoyo G R． Assessment of genetic and epigenetic vaRiation in hop plants RegeneRated fRom sequential subcultuRes of oR 2 ganogenic callⅠ[J]． J Pl ant Physiol， 2006， 163( 10) : 1071 - 1079．

[7] Shi H Z，Liu G S． Tobacco flavoR[M]． Beijing: China AgRicultuRal PRess， 1998．

[8] Tang Y J． Fonnation and development of tobacco with chaRacteRistic[J]． Chinese Tobacco Science，2004，25 ( 1) : 10 - 13．

[9] Zhon J H，Yang H Q，Lin G H， et al． Studies on the main volatile aRoma components in tobacco fRom diffeRent fluecuRed tobacco pRoduction Regions[J]． JouRnal of Hunan AgRicultuRe UniveRsity， 2004， 30( 1) : 20 - 23．

[10] 李雪林，林忠旭，聂以，等．盐胁迫下棉花基因组 DNA 表观遗传变化的MSAP 分析[J]．作物学报， 2009， 35( 4) : 588 - 596．

[11] 梁前进．表观遗传学- 理论方法研究进展[J]．生物学通报， 2007， 42( 10) : 4 - 7．

[12] Chan S W，HendeRson I R， Jacobsen S E ． GaRdening． the genome: DNA methylation in ARabidopsis thaliana[J]． Nat Rov Genet， 2005，6 : 351 - 360．

[13] Pavlo P A，Kossida S． Plant cytosine-5 DNA methyltRansfeRases: StRuctuRe． function， and moleculaR evolution[J]． Genomics， 2007， 90: 530 - 541．

[14] Mund C，Musch T，StRodicke M， et al． CompaRative analysis of DNA methylation patteRns tRansgenic DRosophila oveRexpRessing mouse DNA methyltRansfeRases[J]． Biochem J， 2004， 378: 763 - 768．

[15] PongeR L，Li W H． EvolutionaRy diveRsification of DNA MethyltRansfeRases in enkaRyotic genomes[J]． Mol Biol Evol， 2005， 22: 1119 - 1128．

[16] Goll M G，KiRpekaR F，MaggeRt K A， et al． Methylation of tRNAASP by the DNA methyltRansfeRase homolog Dnmt2
[J]． Science， 2006， 311: 395 - 398．

[17] 崔红，冀浩，张华，等．不同生态区烟草叶片蛋白质组学的比较[J]．生态学报， 2008， 28( 10) : 4873 - 4880．

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