Abstract: The purpose of this experiment was to establish Lm-type female system castor different developmental stages marked female,per female,amphoteric system inforescence establish MSAP reaction system.MSAP reaction system established to determine differences in inflorescence type genes at different developmental stages of research to lay the foundation.The results were as follows:extraction of different types at different developmental stages of inflorescence genomic DNA mixed into the gene pool.EcoRⅠ and HpaⅡ/MspⅠ digested gene pool 12 h.The digestion products and EcoRⅠ,Hpa Ⅱ/MspⅠ adapter overnight connection for pre-amplification.The optimized system for the pre-amplification reaction 10×PCR Buffer 2.5 μL,dNTPs(10 mmol/L)2.5 μL,Mg²⁺(25 mmol/L)2.0 μL,template 2.0 μL,E₀ amplification primer (10 pmol/μL) 1.5 μL,H₀ amplification primer (10 pmol/μL) 1.5 μL,LA Taq(5 U/μL)0.25 μL,ddH₂O 12.75 μL.Pre-amplification products were diluted 10-fold for the selective amplification.Selective amplification system optimized for 10×PCR Buffer 2.5 μL,dNTPs(10 mmol/L)2.0 μL,Mg²⁺(25 mmol/L)4.0 μL,template 3.0 μL,E_X amplification primer(10 pmol/μL)1.0 μL,H/M_x amplification primer(10 pmol/μL)1.0 μL,LA Taq(5 U/μL)0.30 μL,ddH₂O 11.2 μL.

Key words: Castor, Inflorescence, MSAP

CLC Number: 

• S563.03