Determination of Toxic Protein Ricin a Chain Gene was Co-suppression of Seed Poisoning Protein Content

doi:10.7668/hbnxb.2016.01.005

Abstract

Abstract: The purpose of this experiment was to a chain gene toxic protein was co-suppressed in transgenic castor plants T₀,T₁,T₂ seeds were determination of toxic protein content,obtain a low content of toxic protein ricin new transgenic material to lay the foundation.The results were as follows:we used phosphate extraction method,BCA kit method,UVwin5,ultravioletV5.1.0,BandScan protein quantitative analysis.,after obtained the two co-suppression stable genetic material,nameed T₂-9-1,T₂-15,A chain toxin protein content were 0.Contrast Tongliao 5 ricin B chain respectively 77.63%,83.38%,both materials could be used as low-toxic protein ricin material to promote.

Key words: Castor, Ricin, A chain gene

CLC Number:

S565.5

HUANG Fenglan, LEI Xue, ZHAO Yong, LI Guorui, LUO Rui, CHEN Xiaofeng, LI Yue, SUN Huajun. Determination of Toxic Protein Ricin a Chain Gene was Co-suppression of Seed Poisoning Protein Content[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 29-34. doi: 10.7668/hbnxb.2016.01.005.

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http://www.hbnxb.net/EN/Y2016/V31/I1/29

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