cDNA-AFLP Technology Analyze DiffeRentially ExpRessed Genes on DwaRf CastoR Stem

doi:10.7668/hbnxb.2013.06.022

Abstract

Abstract: Screening good genes related to plant height, leaf type and yields in order to culture an internode shorter, caulome stockiness, length medium, and high-yielding dwarf varieties of castor.This paper analyzed differen-tial cDNA fragmentation of dwarf castor caster on date of initial blooming with cDNA-AFLP technology.It gained 128 bands of differences in genes, of which 114 bands can be finded in Balatx database.39 sequences can be anno-tated by GO of blast2GO.These genes are almost covers all kinds of genes related to dwarf involved in cell growth and differentiation, signal transduction, transcription regulation of substance and energy metabolism and transport, etc.They may be plays an important and decisive role in castor dwarf mechanism.And this will find new break-through to reveal the molecular mechanism of castor dwarf.

Key words: CastoR, Stem, cDNA-AFLP, DiffeRential gene

CLC Number:

S565.6

CHEN Yong-sheng, LI Guo-rui, HUANG Feng-lan, WANG Wen-yue. cDNA-AFLP Technology Analyze DiffeRentially ExpRessed Genes on DwaRf CastoR Stem[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 126-129. doi: 10.7668/hbnxb.2013.06.022.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2013.06.022

http://www.hbnxb.net/EN/Y2013/V28/I6/126

References

[1] 郑鹭,祁建民,陈绍军,等.蓖麻遗传育种进展及其在生物能源与医药综合利用潜势[J].中国农学通报,2006,22( 9): 109-113.
[2] 曾祥艳,王东雪,马锦林.我国蓖麻良种选育研究现状及发展策略[J].广西热带农业,2010,6 ( 131): 27 －29.
[3] Yuepeng Song,Zeliang Wang,Wenhao Bo,et al.Transcriptional profiling by cDNA-AFLP analysis showed differential transcript abundance in response to water stress in Populus hopeiensis[J].BMC Genomics,2012,13 ( 286): 1186/1471-2164.
[4] Gupta S,Bharalee R,Bhorali P, et al.Molecular analysis of drought tolerance in tea by cDNA-AFLP based transcript profiling[J].Mol Biotechnol,2013,53( 3): 237 －248.
[5] Gupta N,Naik P K,Chauhan R S.Differential transcript profiling through cDNA-AFLP showed complexity of rutin biosynthesis and accumulation inseedsof a nutraceutical food crop ( Fagopyrum spp.) [J].BMC Genomics,2012,13( 231): 1186/1471-2164.
[6] 王文跃,李国瑞,黄凤兰,等.蓖麻茎秆cDNA-AFLP 反应体系的建立[J].华北农学报, 2013,28( 2): 86-90.
[7] 梁宏伟,王长忠,李忠聚,等.聚丙烯酰胺凝胶快速、高效银染方法的建立[J].遗传,2008,30 ( 10): 1379 －1382.
[8] 萧小鹃,杨远柱,林建中,等.温敏核不育系水稻株1S及其矮秆突变体SV14 茎的蛋白质组学比较[J].高等学校化学学报, 2008,29( 1): 41-45.
[9] 张宽朝,金青,蔡永,等.萍苯丙氨酸解氨酶与其在重要次生代谢产物调控中的作用研究进展[J].中国农学通报, 2008,24( 12): 59-62.
[10] 王萱.辣椒白粉病抗性与苯丙氨酸解氨酶活性的关系[J].中国农学通报, 2009,25( 3): 193-196.
[11] 郭书巧,黄骥,江燕,等.水稻C2H2 型锌指蛋白基因ＲZF71 的克隆与表达分析[J].遗传,2007,29( 5): 607-613.
[12] Gamsjaeger Ｒ,Liew C K,Loughlin F E, et al.Sticky fingers: Zinc-fingers as proten-recognition motifs [J].Trends Biochem Sci,2007,32( 2): 63-70.
[13] 玉晓红,晁丽,陈昌冬,等.利用ＲNA 干涉研究水稻锌指蛋白基因OsZＲL 的功能[J].中国水稻科学,3013,27( 1): 9-16.
[14] 柳忠玉,黄亚东,庄楚雄.参与植物膜泡运输的蛋白质家族及功能研究进展[J].细胞生物学杂志, 2006,28( 3): 447-452.

[1]	WANG Lina, GAO Kang, KANG Chen, TIAN Zhejuan, LI Yadong, WANG Peng, LI Zhaowei, WU Zhiming. Construction of Yeast Library of Tomato Nucleus System and Screening of ToCV CP-interacting Proteins [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 34-43.
[2]	LI Junren, CHEN Xiuzhen, WU Daidi. Cloning,Subcellular Localization,and Expression Analysis of PatASIL2 Gene of Trihelix Family from Pogostemon cablin [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 104-112.
[3]	PENG Keyan, ZHAO Kainan, ZHOU Fabao, DIAO Yanbin, CHEN Guangzhou, LI Shengdong. Effects of Nitrogen Fertilizer Reduction and Postponing on Grain Yield and Nitrogen Absorption and Utilization of Wheat-Maize Double Cropping System [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 133-145.
[4]	XING Baorui, ZHAO Haiping, LI Guangyu, MA Zefang, SUN Hongmei, HU Xiao, TAN Zhanqing, LIU Zhen. Transcriptomic Analysis of PTH 1-34 on the Osteogenesis Mechanism of Antler Mesenchymal Stem Cells [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 344-352.
[5]	DENG Haoyu, WU Yichao, FU Teng, YANG Zaijun, WU Rina. Effects of Cd on Physiological and Biochemical Processes of Wheat CM28 under Glycine Spray [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 63-71.
[6]	LI Junren, WU Daidi, ZHAO Gongzheng, CHEN Xiuzhen. Cloning,Subcellular Localization and Expression Analysis of PatGTL1 Gene of Trihelix Family from Pogostemon cablin [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 84-93.
[7]	LIN Haihong, LU Jiannong, YIN Xuegui, HUANG Guanrong, ZHANG Liuqin, ZHANG Xiaoxiao, LIU Chaoyu, ZUO Jinying. Mapping QTLs Conferring Seed Yield Per Spike and Spike Axis Dry Weight in Castor [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 94-105.
[8]	XIAO Chenyaodong, LIU Tao, LIU Shizhi, ZHANG Shuying. Effect of Exogenous Hydrogen Peroxide on the Physiological Characteristics of Cotton Seedlings under Salt Stress [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 93-101.
[9]	YIN Mingda, LUO Rui, REN Wenjing, WANG Zhiyan, SU Zhimin, LI Ruxin, CHEN Zhen, LI Yuling, WANG Yan, HUANG Fenglan. *Cloning and Functional Prediction of Castor PIP5K2* Gene** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 72-79.
[10]	WU Xincheng, HE Risheng, XIAO Shuoding, ZHANG Zhenqian, YANG Liu, LIU Zhongsong, CHEN Hao. Identification and Candidate Gene Mining of Stem Lodging Resistance in Brassica napus [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 54-63.
[11]	ZHENG Wenzhe, WANG Yingying, ZHANG Hui, ZHANG Bizhou, SUN Mengyuan, WANG Liang, ZHANG Huizhong, LI Xiaodong, FU Zengjuan, ZHAO Shangmin, E Yuanyuan, ZHANG Ziqiang. Effects of Nano-selenium on the Transcriptome of Sugar Beet Leaves [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 51-59.
[12]	LI Yanxiao, WANG Lina, ZHU Guishuang, LIU Peng, XIANG Dianjun. *Cloning and Expression Characteristics Analysis of RcNAC100-like* Gene in Castor** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 67-76.
[13]	ZHANG Xiaoxiao, YIN Xuegui, LU Jiannong, HUANG Guanrong, ZHANG Liuqin, LIU Chaoyu, LIN Haihong, ZUO Jinying. QTL Mapping and Candidate Gene Analysis for Harvest Index and Related Traits in Castor [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 77-87.
[14]	GUO Yuanhang, WANG Hongbo, BAI Baowei, ZHANG Lei, ZHAO Fengnian, LÜ Dongxue, JIA Ting, WANG Xingpeng. Effects of Exogenous Melatonin on Salt Stress Reduction in Soybean Seedlings [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 116-125.
[15]	SHEN Jinwei, LU Jianwei, ZHAO Xue, ZHA Lao, CHENG Shuru, LIANG Chunnian. *Cloning,Bioinformatics,and Tissue Expression Analysis of Yak DJ-1* Gene** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 192-199.

Please choose a citation manager

Content to export